Predicted Species Reactivity:HumanApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology PCSK4 ELISA Kit (Human) (OKEH07661) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCSK4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PCSK4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PCSK4 captured in well.Kit Range:78 - 5000pg/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=9.1% (n="">Mean Inter-assay CV%: <=11.8% (n="">Kit Component:| Component | Amount |
|---|
| PCSK4 Microplate | 96 Wells (12 x 8 Well strips) |
| PCSK4 Lyophilized Standard | 2 |
| 100X Biotinylated PCSK4 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Proprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues (By similarity). In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida (By similarity). Plays also a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2 (PubMed:16040806). May also participate in folliculogenesis in the ovaries.:::Subcellular Location: Membrane Single-pass membrane protein Cytoplasmic vesicle Secretory vesicle Acrosome membrane:::Unigene: Hs.46884SMR: Q6UW60String: 9606.ENSP00000300954:::KEGG: hsa:54760MIM: 600487Pfam: PF01483:::KEGG: hsa:54760MIM: 600487Pfam: PF01483Reconstitution and Storage:Store as indicated in product manual.Sensitivity:22pg/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Human Proprotein convertase subtilisin/kexin type 4Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:PCSK4Official Gene Full Name:proprotein convertase subtilisin/kexin type 4Alias Symbols:PC4, SPC5NCBI Gene Id:54760Protein Name:proprotein convertase subtilisin/kexin type 4Description of Target:This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. The protease is expressed only in the testis, placenta, and ovary. It plays a critical role in fertilization, fetoplacental growth, and embryonic development and processes multiple prohormones including pro-pituitary adenylate cyclase-activating protein and pro-insulin-like growth factor II.Swissprot Id:Q6UW60Protein Accession #:NP_060043.2Nucleotide Accession #:NM_017573.4Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express PCSK4. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express PCSK4.
