Methylcholanthrene Antibody (OADC00002)

货号：	OADC00002
抗体英文名：	MethylcholanthreneAntibody(OADC00002)
应用范围：	DB
宿主：	Rabbit
适应物种：	Human
标记物：	Unconjugated
克隆性：	否
保存条件：	Storeat-20C.Avoidrepeatedfreeze/thawcycles.
形态：	Liquid.ProvidedinPBScontaining0.05%azideand0.05%ProClin300.
规格：	50ug

3-methylcytosinearisesbyerroneousmethylationofDNAthroughspontaneousexposuretoendogenousS-adenosylmethionine(SAM).SincetheN3-positionincytosineisprotectedindoublestrandedDNA,thisphenomenonmainlyoccursinsinglestrandedDNA.Theresulting3-mCistoxicandmutagenicandincreasedlevelsof3-mCseemtoimpaircellproliferation.Therefore,ithastoberepairedbythecell.Thisisestablishedbyoxidativedemethylation,whichiscatalyzedbytheAlkBprotein.-Seemoreat:http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OADC00002

3-mCr

DotblotanalysisusingtheAvivaantibodydirectedagainst3-mCr.TodemonstratethespecificityoftheAvivaantibodyagainst3-mC,aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedbases.150and50pmoloftherespectiveoligoswerespottedonthemembrane.Theantibodywasdiluted1:400inPBS-Tcontaining10%skimmilkand1%BSA.Figureshowsahighspecificityoftheantibodyfortheoligonucleotidewiththe3-methylcytosinemodification.