Gene Symbols:CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A| IL1A | IL1B | IL2 | IL3 | IL4 | IL5 | IL6 | IL9 | TNF | VEGFA View more Hide
Number of Targets Detected:20
Compatible Sample Types:- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Method of Detection:Fluorescence Laser Scanner
Quantitative/Semi-Quantitative: Solid Support:Glass Slide
Size:1, 2, or 4 Slide Kit (16 sub-arrays per slide)
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Scroll over each target protein for more information |
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GM-CSF- Gene Symbol: CSF2
- Accession #: P26955
- ELISA Kit: RayBio Mouse GM-CSF ELISA
| IFN-gamma- Gene Symbol: IFNG
- Accession #: P01580
- ELISA Kit: RayBio Mouse IFN-gamma ELISA
| IL-1 alpha(IL-1 F1)- Gene Symbol: IL1A
- Accession #: P01582
- ELISA Kit: RayBio Mouse IL-1 alpha ELISA
| IL-1 beta(IL-1 F2)- Gene Symbol: IL1B
- Accession #: P10749
- ELISA Kit: RayBio Mouse IL-1 beta ELISA
| IL-10- Gene Symbol: IL10
- Accession #: Q0VBJ1
- ELISA Kit: RayBio Mouse IL-10 ELISA
|
IL-12 p70- Gene Symbol: IL12A
- Accession #: P43431
| IL-13- Gene Symbol: IL13
- Accession #: P20109
- ELISA Kit: RayBio Mouse IL-13 ELISA
| IL-17A- Gene Symbol: IL17A
- Accession #: Q60971
- ELISA Kit: RayBio Mouse IL-17A ELISA
| IL-2- Gene Symbol: IL2
- Accession #: Q791T3
- ELISA Kit: RayBio Mouse IL-2 ELISA
| IL-3- Gene Symbol: IL3
- Accession #: P01586
- ELISA Kit: RayBio Mouse IL-3 ELISA
|
IL-4- Gene Symbol: IL4
- Accession #: P07750
- ELISA Kit: RayBio Mouse IL-4 ELISA
| IL-5- Gene Symbol: IL5
- Accession #: P04401
- ELISA Kit: RayBio Mouse IL-5 ELISA
| IL-6- Gene Symbol: IL6
- Accession #: P08505
- ELISA Kit: RayBio Mouse IL-6 ELISA
| IL-9- Gene Symbol: IL9
- Accession #: P15247
- ELISA Kit: RayBio Mouse IL-9 ELISA
| KC(CXCL1)- Gene Symbol: CXCL1
- Accession #: P12850
- ELISA Kit: RayBio Mouse KC ELISA
|
MCP-1(CCL2)- Gene Symbol: CCL2
- Accession #: Q9QYD7
- ELISA Kit: RayBio Mouse MCP-1 ELISA
| M-CSF- Gene Symbol: CSF1
- Accession #: P07141
- ELISA Kit: RayBio Mouse M-CSF ELISA
| RANTES(CCL5)- Gene Symbol: CCL5
- Accession #: P30882
- ELISA Kit: RayBio Mouse RANTES ELISA
| TNF alpha- Gene Symbol: TNF
- Accession #: Q62326
- ELISA Kit: RayBio Mouse TNF-alpha ELISA
| VEGF-A- Gene Symbol: VEGFA
- Accession #: Q00731
- ELISA Kit: RayBio Mouse VEGF-A ELISA
|
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.