产品资料

RayBiotech/Human Inflammation Array Q3/8 Sample Kit/QAH-INF-3-1

Antigen Information

Gene Symbols:

CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CSF1 | CSF2 View more Hide

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
BLC(CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	Eotaxin-1(CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2(MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA
I-309(TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	ICAM-1(CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-1 alpha(IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta(IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA
IL-1 ra(IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA
IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8(CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA
IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA
IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552	MCP-1(CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MIG(CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 alpha(CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta(CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta(CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	RANTES(CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA
TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta(TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TNF RI(TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TNF RII(TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Inflammation Array Q3 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024. Species: HumanSample type: Conditioned Media
Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.Species: HumanSample type: Serum
Liao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907. Species: HumanSample type: Conditioned Media
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327Species: HumanSample type: Conditioned Media
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.Species: HumanSample type: Conditioned Media
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094Species: HumanSample type: Conditioned Media
Wang S., Lu Y., Hong Q., et al. Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy. Biomed Res Int. 2017; 2017: 7843584. doi: 10.1155/2017/7843584Species: HumanSample type: Tissue Lysate
Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.Species: HumanSample type: Vitreous Fluid
Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.Species: HumanSample type: Plasma
Gu R., Zhou M., Jiang C., et al. Elevated concentration of cytokines in aqueous in post-vitrectomy eyes. Clin Exp Ophthalmol. 2016 Mar;44(2):128-34. doi: 10.1111/ceo.12638Species: HumanSample type: Aqueous Humor

123

My lab members have used this array several times before, they recommend it to me, both mouse's and human's are very reliable!

Xue HaoThe Wistar Institute11/21/2019, 07:38 PM

It's good and easy to use, but need a laser scanner.

Xingmin SunUniversity of South Florida09/19/2018, 10:03 AM

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Manual

SDS

GAL

Analysis Tool

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