产品资料

RayBiotech/Human Cytokine Array Q1000/8 Sample Kit/QAH-CAA-1000-1

Antigen Information

Gene Symbols:

AREG | BDNF | BMP4 | BMP5 | BMP7 | CCL1 | CCL11 | CCL15 | CCL2 | CCL24 View more Hide

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 100 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC(CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA
BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA	BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA
Eotaxin-1(CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2(MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA	FGF-7(KGF) Gene Symbol: FGF7 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA
GDF-15 Gene Symbol: GDF15 Gene ID: 9518 Accession #: Q99988 ELISA Kit: RayBio Human GDF-15 ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	EG-VEGF(PK1) Gene Symbol: PROK1 Gene ID: 84432 Accession #: P58294 ELISA Kit: RayBio Human EG-VEGF ELISA
HB-EGF Gene Symbol: HBEGF Gene ID: 1839 Accession #: Q99075 ELISA Kit: RayBio Human HB-EGF ELISA	HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309(TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	ICAM-1(CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA
IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IL-1 alpha(IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta(IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 ra(IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA
IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA
IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17A ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8(CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	Insulin Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human Insulin ELISA
MCP-1(CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	M-CSF R Gene Symbol: CSF1R Gene ID: 1436 Accession #: P07333 ELISA Kit: RayBio Human M-CSF R ELISA	MIG(CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 alpha(CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta(CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta(CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	NGFR(TNFRSF16) Gene Symbol: NGFR Gene ID: 4804 Accession #: P08138 ELISA Kit: RayBio Human NGF R ELISA	NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA	NT-4 Gene Symbol: NTF4 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA
Osteoprotegerin(TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PDGF-AA Gene Symbol: PDGFA Gene ID: 5154 Accession #: P04085 ELISA Kit: RayBio Human PDGF-AA ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	RANTES(CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA
SCF Gene Symbol: KITLG Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SCF R(CD117/c-kit) Gene Symbol: KIT Gene ID: 3815 Accession #: P10721 ELISA Kit: RayBio Human SCF R ELISA	TGF alpha Gene Symbol: TGFA Gene ID: 7039 Accession #: P01135 ELISA Kit: RayBio Human TGF-alpha ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600
TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta(TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TNF RI(TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII(TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human VEGF R3 ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range

Kit Components

Human Cytokine Array Q1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Massee M., Chinn K., Lim J., et al. Type I and II Diabetic Adipose-Derived Stem Cells Respond In Vitro to Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion. Adv Wound Care (New Rochelle). 2016 Feb 1;5(2):43-54 Species: HumanSample type: Conditioned Media
Ciavarella S., Caselli A., Tamma A., et al. A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression. Stem Cells Dev. 2015 Jun 15;24(12):1457-70. doi: 10.1089/scd.2014.0254. Species: HumanSample type: Conditioned Media
Massee M., Chinn K., Lei J., et al. Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro. J Biomed Mater Res B Appl Biomater. 2015 Jul 14. doi: 10.1002/jbm.b.33478Species: HumanSample type: Conditioned Media

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