产品资料

RayBiotech/Human Autoimmune Disease IgG Autoantibody Array G1/16 Sample Kit/PAH-AIDG-G1-16

Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Serum

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

16 Sample Kit

Product Features

Small sample volume required (2 µl)
High density (simultaneously detect 33 autoantibodies)
High sensitivity
Large dynamic range
Suitable for high-throughput assays
High efficiency and accuracy
Affordable, quick and simple to use
Customizable: Tell us the autoimmunogens you"re interested in

Target Name

Scroll over each target protein for more information
Alanyl-tRNA Synthetase	Cytokeratin 8(KRT8) Gene Symbol: KRT8 CYK8 Gene ID: 3856 Accession #: P05787 ELISA Kit: RayBio Human Cytokeratin 8/KRT8 ELISA	Centromere protein B(CENP-B) Gene Symbol: CENPB Gene ID: 1852 Accession #: P07199	Centromere protein A(CENP-A) Gene Symbol: CENPA Gene ID: 1851 Accession #: P49450	dsDNA
Gliadin	Glycine-tRNA ligase(AP-4-A synthetase/GlyRS) Gene Symbol: GARS Gene ID: 4162 Accession #: P41250	Nuclear pore protein gp210(POM210/Nucleoporin Nup210) Gene Symbol: NUP210 KIAA0906 PSEC0245 Gene ID: 30052 Accession #: Q8TEM1	Histone H2A	Histone H2B
Cytokeratin 19(KRT19) Gene Symbol: KRT19 Gene ID: 3880 Accession #: P08727 ELISA Kit: RayBio Human Cytokeratin 19/KRT19 ELISA	Histidine-tRNA ligase(HisRS) Gene Symbol: HARS Gene ID: 4816 Accession #: P12081	Lupus La protein(SS-B) Gene Symbol: SSB Gene ID: 11316 Accession #: P05455	LC-1	Cytokeratin 20(KRT20) Gene Symbol: KRT20 Gene ID: 54474 Accession #: P35900 ELISA Kit: RayBio Human Cytokeratin 20 ELISA
M/Scl 100	M2	Mi-2	Myeloperoxidase Gene Symbol: MPO Gene ID: 4353 Accession #: P05164	Nuclear pore glycoprotein p62(Nucleoporin Nup62) Gene Symbol: NUP62 Gene ID: 8066 Accession #: P37198
Calcitonin Gene Symbol: CALCA CALC1 Gene ID: 796 Accession #: P01258 ELISA Kit: RayBio Human Calcitonin ELISA	PCNA Gene Symbol: PCNA Gene ID: 5111 Accession #: P12004 ELISA Kit: RayBio Human PCNA ELISA	Myeloblastin(PR3/C-ANCA) Gene Symbol: PRTN3 MBN Gene ID: 9495 Accession #: P24158	TRIM21(Ro(SS-A)/SS-A) Gene Symbol: TRIM21 RNF81 RO52 SSA1 Gene ID: 11312 Accession #: P19474	TRIM21(Ro(SS-A)/SS-A) Gene Symbol: TRIM21 RNF81 RO52 SSA1 Gene ID: 11312 Accession #: P19474
DNA topoisomerase 1(DNA topoisomerase I) Gene Symbol: TOP1 Gene ID: 11986 Accession #: P11387	Sm core protein	Sp-100 Gene Symbol: SP100 Gene ID: 11206 Accession #: P23497	Threonine-tRNA ligase(ThrRS) Gene Symbol: TARS2 Gene ID: 30740 Accession #: Q9BW92	Thyroglobulin Gene Symbol: TG Gene ID: 7038 Accession #: P01266 ELISA Kit: RayBio Human Thyroglobulin ELISA
Thrombopoietin(TPO) Gene Symbol: THPO MGDF Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	Transglutaminase 2(TGM2) Gene Symbol: TGM2 Gene ID: 7052 Accession #: P21980 ELISA Kit: RayBio Human Transglutaminase 2 ELISA	snRNP70 Gene Symbol: SNRNP70 RNPU1Z RPU1 SNRP70 U1AP1 Gene ID: 11150 Accession #: P08621

Application Notes

Suggested Application

IgG autoantibody profiling for following common autoimmune diseases, but may not be limited to:

Systemic lupus erythematosus (SLE)
Sjøgren"s syndrome
Rheumatoid arthritis
Systemic Sclerosis/CREST syndrome
Primary biliary cirrhosis
Autoimmune hepatitis
Hashimoto"s thyroiditis
Polymyositis/Dermatomyositis
System vasculitis
Celiac disease

The following table shows multiple IgG-type autoantibodies that can be detected in patient sera of different autoimmune diseases.

Autoimmune Diseases	Target No.	Autoimmunogens
Systemic lupus erythematosus (SLE)	#5	dsDNA
	#9	Histone H2A
	#10	Histone H2B
	#22	Proliferating Cell Nuclear Antigen (PCNA)
	#26	Scl-70 (DNA topoisomerase-1)
	#33	U1-snRNP70 kDa
	#12	Jo-1 (histidyl tRNA synthetase, histidine-tRNA ligase)
	#27	Sm/Smith/snRNP core protein
Rheumatoid arthritis	#21	CCP peptide
	#2	KRT8
	#11	KRT19
	#15	KRT20
Systemic Sclerosis / CREST Syndrome	#4	Centromere Protein A (CENP-A)
	#3	Centromere B (CENP-B)
	#16	M/Scl 100
	#27	Sm/Smith/snRNP core protein
Primary biliary cirrhosis	#8	gp-210
	#17	M2
	#20	Nup62
	#28	sp100
Autoimmune hepatitis	#14	Liver cystolic antigen type 1 (LC-1)
Hashimoto"s thyroiditis	#30	Thyroglobulin (TG)
Hashimoto"s thyroiditis	#31	Thyroid peroxidase (TPO)
Polymyositis / Dermatomyositis	#1	Alanyl-tRNA Synthetase (PL-12)
	#7	Glycyl-tRNA Synthetase (EJ)
	#18	Mi-2
	#29	Threonyl-tRNA synthetase (PL-7)
	#14	Liver cystolic antigen type 1 (LC-1)
System vasculitis	#19	Myeloperoxidase (MPO), p-ANCA
	#23	Proteinase-3 (PR3), c-ANCA
	#3	Centromere B (CENP-B)
Sjøgren"s syndrome	#13	La/SS-B
	#24	Ro/SS-A (52 kDa)
	#25	Ro/SS-A (60 kDa)
Celiac disease	#6	Gliadin
Celiac disease	#32	Tissue Transglutaminase (tTG)

Kit Components

RayBio^® Human Autoimmune Disease IgG Autoantibody G1 array slide
RayBio^® G-series antibody array accessory (including slide incubation chamber assembly, gasket, protective cover, snap-on sides and adhesive film)
Blocking Buffer (8 ml/bottle)
Wash Buffer 1 (30 ml/bottle)
Wash Buffer 2 (30 ml/bottle)
1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
1,500X Streptavidin-Conjugated Fluor (1 µl/vial)

Other Materials Required

Small plastic boxes or containers
Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Distilled water
Aluminum foil
Laser scanner for fluorescence detection

Protocol Outline

See Manual for relevant protocols.

Below is the quick protocol for the experienced users.

The quick protocol for the experienced users.

Typical Data

The following Figure 1 represents a typical result using serum on the RayBio^® Autoimmune Disease IgG Autoantibody Detection Array (Cat #. PAH-AIDG-G1). The protein arrays were incubated with 200-fold diluted serum samples from normal individuals and patients with different autoimmune diseases. After washing steps, the arrays were sequentially incubated with biotin-labeled anti-human IgG antibody and fluorescent dye-labeled streptavidin. The signals from the bound autoantibodies were captured with a fluorescence scanner.

Sample diluent alone (Figs. 1A & 1B) and normal sera (Figs. 1C & 1D) were used as negative controls, and the arrays signals indicate that no significant background occurred. In some cases, a negligible signal can be observed, which may be caused by non-specific binding derived from the biotin-labeled anti-human IgG antibody and/or fluorescent dye-labeled streptavidin. However, these weak background signals will be subtracted in subsequent data analysis procedures and will not affect sample test results. A strong binding signal was detected in multiple targets among different autoimmune disease patients: systemic lupus erythematosus (Figs.1E & 1F), Sjøgren"s syndrome (Figs.1G & 1H), system vasculitis (Figs.1I & 1J), and primary biliary cirrhosis (Figs.1K & 1L).

Typical result using serum on the RayBio<sup>®</sup> Autoimmune Disease IgG Autoantibody Detection Array.

Fig 1. Typical result using serum on the RayBio^® Autoimmune Disease IgG Autoantibody Detection Array. The protein arrays were incubated with diluent alone (A, B), diluted serum samples derived from normal individuals (C, D) and those from various autoimmune disease patients (E-L). Yellow boxes, show various controls as indicated (biotin-labeled BSA, human IgG and IgA).

Storage/Stability

Upon receipt, all components of the RayBio^® Human Protein Array kit should be stored at -20 ^°C to -80 ^°C until just before the experiment. At -20 ^°C to -80 ^°C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at -20 ^°C and all other component should be stored at 4 ^°C. Use within three months after reagents have been thawed.

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