Description
Fuc123 Alpha-L-Fucosidase is thermostable enzyme that cleaves alpha-glucan residues from various alpha-fucosylated oligosaccharides including hydrolysis of alpha-L-fucose from algal fucosidan (Ascophyllum nodosum).
The enzyme is available for purchase as 30 units of enzyme.
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Enzyme activity: Fuc123 Alpha-Fucosidase is a glycosyl hydrolase that cleaves alpha-glucan residues from various alpha-fucosylated oligosaccharides including hydrolysis of alpha-L-fucose from algal fucosidan (Ascophyllum nodosum).
Activity determination: The standard assay is performed by mixing 10 μL enzyme sample to 80 μL of 125mM phosphate buffer at pH 6,5 with 20 μL PNP-alpha-fucopyranoside, incubated at 65°C. Reaction is stopped with 200μL 0,5M sodium borat and OD measured at 405 nm.
Unit definition: One unit of activity is defined as the amount of enzyme, which liberates 1 μmol of p-nitrophenol per minute under the given assay conditions.
Synonyms: glycoside hydrolase, alpha-L-fucoside fucohydrolase, alpha-fucosidase
Protein family: Glycosyl hydrolase family
Enzyme classification: EC 3.2.1.51
Source: Proprietary environmental DNA
Substrates:
Fucoidan is a group of sulfated polysaccharides containing fucose and includes molecules with a backbone built of (1→3)-linked alpha-L-fucopyranosyl or alternating (1→3)- and (1→4)-linked alpha-L-fucopyranosyl residues, but also includes sulfated galactofucans with backbones built of (1→6)-beta-D-galacto- and/or (1→2)-beta-D-mannopyranosyl units and may include branching and various substitutions of sugar residues. Fucoidan from seaweed has found use as dietary supplement and has been implicated as having potential bioactive functions in humans ( Ale et al 2011 ).
Properties of ThermoActive™ Alpha-Fucosidase:
Temperature Optimum: The enzyme in relatively active in a temperature range (60-70°C)with optimum around 65°C
pH Optimum: suitable pH range is about 6-7 with optimum around 6.5
References: