产品资料

Sino Biological/AGO1 Protein, Human, Recombinant (His Tag)/1/11225-H07B

Description
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Protein Expression Service

AGO1 Protein, Human, Recombinant (His Tag): Product Information

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the full length of human AGO1 (NP_036331.1) (Met 1-Ala 857) was expressed, with a polyhistidine tag at the N-terminus.

Accession#

NP_036331.1

Expressed Host

Baculovirus-Insect Cells

Species

Human

Predicted N Terminal

His

Molecule Mass

The recombinant human AGO1 consists of 875 amino acids and predicts a molecular mass of 99.5 kDa as estimated in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

AGO1 Protein, Human, Recombinant (His Tag): Images

AGO1 Protein, Human, Recombinant (His Tag): Synonyms

EIF2C Protein, Human; EIF2C1 Protein, Human; GERP95 Protein, Human; Q99 Protein, Human

AGO1 Background Information

Protein argonaute-1, also known aseukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and agosubfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. Thisgene is located on chromosome 1 in a cluster of closely related family membersincluding argonaute 3, and argonaute 4. This genomic region is frequently lostin human cancers such as Wilms tumors, neuroblastoma, and carcinomas of thebreast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed atlow to medium levels. Differential polyadenylation and splicing result in acomplex transcriptional pattern.EIF2C1 protein contains onePAZdomain and onePiwi domain. It is required for RNA-mediated gene silencing(RNAi) and transcriptional gene silencing (TGS) of promoter regions which arecomplementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAssuch as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and repressesthe translation of mRNAs which are complementary to them.

Full Name

argonaute RISC catalytic component 1

References

Koesters R., et al.,(1999), Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. Genomics 61:210-218.
Gregory S.G., et al., (2006), The DNA sequence and biological annotation of human chromosome 1.Nature 441:315-321.
Motamedi M.R., et al.,(2004), Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell. 119:789-802.

Quantitative functions of Argonaute proteins in mammalian development
Author
Wang, D;Zhang, Z;O"Loughlin, E;Lee, T;Houel, S;O"Carroll, D;Tarakhovsky, A;Ahn, NG;Yi, R;
Year
2012
Journal
Genes Dev.
PubMed ID: 22474261FoldedExpand
DNA Tetrahedron Based Biosensor for Argonaute2 Assay in Single Cells and Human Immunodeficiency Virus Type-1 Related Ribonuclease H Detection in Vitro
Author
Zhang, K;Huang, W;Huang, Y;Li, H;Wang, K;Zhu, X;Xie, M;
Year
2019
Journal
Anal. Chem.
Application
binding (DNA)
PubMed ID: 31050888FoldedExpand
A well-designed Gold nanoparticle based fluorescence probe for assay Argonaute2 and Let-7a interaction in living cells
Author
Zhang, K;Fan, Z;Huang, Y;Xie, M;Zhao, J;Wang, J;
Year
2020
Journal
Sensors and Actuators B: Chemical
Application
binding
FoldedExpand

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Sino Biological/AGO1 Protein, Human, Recombinant (His Tag)/1/11225-H07B

Description

Related Products

Citations

Protein Expression Service

AGO1 Protein, Human, Recombinant (His Tag): Product Information

AGO1 Protein, Human, Recombinant (His Tag): Images

AGO1 Protein, Human, Recombinant (His Tag): Synonyms

AGO1 Background Information

Citation List

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