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Sino Biological/CD8 alpha Protein, Mouse, Recombinant (His Tag)/1/50389-M08H

Description
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Citations
Protein Expression Service

CD8 alpha Protein, Mouse, Recombinant (His Tag): Product Information

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">

Activity

1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA.2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA.3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.

Protein Construction

A DNA sequence encoding the extracellular domain of mouse CD8a (NP_001074579.1) (Met 1-Tyr 196) was expressed, with a C-terminal polyhistidine tag.

Accession#

NP_001074579.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Lys 28

Molecule Mass

The secreted recombinant mouse CD8a consists of 180 amino acids and has a calculated molecular mass of 20.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 35 kDa protein in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD8 alpha Protein, Mouse, Recombinant (His Tag): Images

CD8 alpha Protein, Mouse, Recombinant (His Tag): Synonyms

BB154331 Protein, Mouse; Ly-2 Protein, Mouse; Ly-35 Protein, Mouse; Ly-B Protein, Mouse; Lyt-2 Protein, Mouse

CD8 alpha Background Information

T-cell surface glycoprotein CD8alpha chain, also known as CD8a, is a single-pass type I membrane protein. TheCD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer(NK) cells and has been implicated in the recognition of monomorphicdeterminants on major histocompatibility complex (MHC) Class I antigens, and insignal transduction during the course of T-cell activation. Both human androdent CD8 antigens are comprised of two distinct polypeptide chains, alpha andbeta. The Ig domains of CD8 alpha are involved in controlling the ability ofCD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alphareduced the ability of the protein to fold properly and, therefore, to beexpressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8deficiency is a novel autosomal recessive immunologic defect characterized byabsence of CD8+ cells, leading to recurrent bacterial infections.

Full Name

CD8a molecule

References

ReferencesDevine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

Human CD8 alpha Proteins

CD8 alpha Protein, Human, Recombinant (His Tag)10980-H08H
CD8 alpha Protein, Human, Recombinant (His Tag), Biotinylated10980-H08H-B
CD8 alpha Protein, Human, Recombinant (ECD, hFc Tag)10980-H02H

Mouse CD8 alpha Proteins

CD8 alpha Protein, Mouse, Recombinant (hFc Tag)50389-M02H
CD8 alpha Protein, Mouse, Recombinant (His Tag), Biotinylated50389-M08H-B

Rat CD8 alpha Proteins

CD8 alpha Protein, Rat, Recombinant (hFc Tag)80285-R02H

Cynomolgus CD8 alpha Proteins

CD8 alpha Protein, Cynomolgus, Recombinant (ECD, hFc Tag)90888-C02H

Ferret CD8 alpha Proteins

CD8 alpha Protein, Ferret, Recombinant (His Tag)60001-F08H

Functional ligands to target molecules
Author
GW Jackson
Year
2013
Journal
US Patent App. 13/748,566
FoldedExpand

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Sino Biological/CD8 alpha Protein, Mouse, Recombinant (His Tag)/1/50389-M08H

Description

Related Products

Citations

Protein Expression Service

CD8 alpha Protein, Mouse, Recombinant (His Tag): Product Information

CD8 alpha Protein, Mouse, Recombinant (His Tag): Images

CD8 alpha Protein, Mouse, Recombinant (His Tag): Synonyms

CD8 alpha Background Information

Human CD8 alpha Proteins

Mouse CD8 alpha Proteins

Rat CD8 alpha Proteins

Cynomolgus CD8 alpha Proteins

Ferret CD8 alpha Proteins

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