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Sino Biological/beta-Actin cDNA ORF Clone, Human, N-GFPSpark® tag/1/HG10962-ANG

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beta-Actin cDNA ORF Clone, Human, N-GFPSpark® tag: General Information

Gene

Species

Human

NCBI Ref Seq

NM_001101.3

RefSeq ORF Size

1128 bp

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human actin, beta with N terminal GFPSpark tag.

Plasmid

Promoter

Enhanced CMV promoter

Vector

pCMV3-N-GFPSpark®

Restriction Sites

KpnI + XbaI (6kb + 1.86kb)

Tag Sequence

GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG

Sequencing Primers

T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

beta-Actin cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

beta-Actin cDNA ORF Clone, Human, N-GFPSpark® tag: Validated Images

Human beta Actin/ACTB Gene Expression validated Image 671

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

beta-Actin cDNA ORF Clone, Human, N-GFPSpark® tag: Synonyms

BRWS1 cDNA ORF Clone, Human; PS1TP5BP1 cDNA ORF Clone, Human

beta-Actin Background Information

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.

Full Name

actin, beta

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beta-Actin cDNA ORF Clone, Rat,Related Products

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Rat beta-Actin qPCR Primer PairRP301046

Intracellular hydrogelation preserves fluid and functional cell membrane interfaces for biological interactions
Author
Lin, JC;Chien, CY;Lin, CL;Yao, BY;Chen, YI;Liu, YH;Fang, ZS;Chen, JY;Chen, WY;Lee, NN;Chen, HW;Hu, CJ;
Year
2019
Journal
Nat Commun
PubMed ID: 30837473FoldedExpand

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