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Sino Biological/TREM-2 Protein, Mouse, Recombinant (His Tag)/1/50149-M08H

Description
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Citations
Protein Expression Service

TREM-2 Protein, Mouse, Recombinant (His Tag): Product Information

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the mouse TREM2 (NP_112544.1) extracellular domain (Met 1-Pro 168) was expressed, with a C-terminal polyhistidine tag.

Accession#

NP_112544.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Leu 19

Molecule Mass

The recombinant mouse TREM2 consists of 161 amino acids with the predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rmTREM2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TREM-2 Protein, Mouse, Recombinant (His Tag): Images

TREM-2 Protein, Mouse, Recombinant (His Tag): Synonyms

TREM-2 Protein, Mouse; Trem2a Protein, Mouse; Trem2b Protein, Mouse; Trem2c Protein, Mouse

TREM-2 Background Information

Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.

Full Name

triggering receptor expressed on myeloid cells 2

References

Bouchon,A. et al., 2000, J. Immunol. 164: 4991-4995.
Paloneva,J. et al., 2002, Am. J. Hum. Genet. 71:656-662.
Prada,I. et al., 2006, Neuroscience. 140(4): 1139-48.
Neumann,H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9.
Thrash,JC. et al., 2009, Neurochem Res. 34(1): 38-45.

Human TREM-2 Proteins

TREM-2 Protein, Human, Recombinant (ECD, His Tag)11084-H08H

Mouse TREM-2 Proteins

TREM-2 Protein, Mouse, Recombinant (hFc Tag)50149-M02H

The Alzheimer"s disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway
Author
Jendresen, C;Årskog, V;Daws, MR;Nilsson, LN;
Year
2017
Journal
J Neuroinflammation
Application
binding
PubMed ID: 28320424FoldedExpand
Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region
Author
Schlepckow, K;Monroe, KM;Kleinberger, G;Cantuti-Castelvetri, L;Parhizkar, S;Xia, D;Willem, M;Werner, G;Pettkus, N;Brunner, B;Sülzen, A;Nuscher, B;Hampel, H;Xiang, X;Feederle, R;Tahirovic, S;Park, JI;Prorok, R;Mahon, C;Liang, CC;Shi, J;Kim, DJ;Sabelström, H;Huang, F;Di Paolo, G;Simons, M;Lewcock, JW;Haass, C;
Year
2020
Journal
EMBO Mol Med
PubMed ID: 32154671FoldedExpand

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Sino Biological/TREM-2 Protein, Mouse, Recombinant (His Tag)/1/50149-M08H

Description

Related Products

Citations

Protein Expression Service

TREM-2 Protein, Mouse, Recombinant (His Tag): Product Information

TREM-2 Protein, Mouse, Recombinant (His Tag): Images

TREM-2 Protein, Mouse, Recombinant (His Tag): Synonyms

TREM-2 Background Information

Human TREM-2 Proteins

Mouse TREM-2 Proteins

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