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Sino Biological/Transthyretin Protein, Human, Recombinant (His Tag)/1/12091-H08H

Description
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Protein Expression Service

Transthyretin Protein, Human, Recombinant (His Tag): Product Information

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">

Activity

Measured by its binding ability in a functional ELISA.Immobilized recombinant human TTR-His (Cat:12091-H08H）at 10 μg/ml (100 μl/well) can bind recombinant Canine RBP4-Fc (Cat:70006-D02H) with a linear range of 0.3-10.0 μg/ml.

Protein Construction

A DNA sequence encoding the human TTR (NP_000362.1) (Met 1-Glu 147) with a C-terminal polyhistidine tag was expressed.

Accession#

NP_000362.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gly 21

Molecule Mass

The secreted recombinant human TTR comprises 138 amino acids with a predicted molecular mass of 15.2 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Transthyretin Protein, Human, Recombinant (His Tag): Images

Human Prealbumin/Transthyretin Protein 9963

Measured by its binding ability in a functional ELISA. Immobilized recombinant human TTR-His (Cat: 12091-H08H）at 10 μg/ml (100 μl/well) can bind recombinant Canine RBP4-Fc (Cat: 70006-D02H) with a linear range of 0.3-10.0 μg/ml.

Transthyretin Protein, Human, Recombinant (His Tag): Synonyms

CTS Protein, Human; CTS1 Protein, Human; HEL111 Protein, Human; HsT2651 Protein, Human; PALB Protein, Human; TBPA Protein, Human

Transthyretin Background Information

Prealbumin/Transthyretin, also known as ATTR, Prealbumin, TTR and PALB, is a secreted and cytoplasm protein that belongs to the Prealbumin / Transthyretin family. Prealbumin / Transthyretin is detected in serum and cerebrospinal fluid (at protein level). It is highly expressed in choroid plexus epithelial cells. It is also detected in retina pigment epithelium and liver. Each monomer of Prealbumin / Transthyretin has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. Prealbumin/Transthyretin is a carrier protein. It transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. Defects in Prealbumin / Transthyretin are the cause of amyloidosis type 1 (AMYL1) which is a hereditary generalized amyloidosis due to Prealbumin / Transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.

Full Name

transthyretin

References

Westermark P, et al. (1990) Fibril in senile systemic amyloidosis is derived from normal transthyretin. Proc Natl Acad Sci U S A. 87(7): 2843-5.
Colon W, et al. (1992) Partial denaturation of transthyretin is sufficient for amyloid fibril formation in vitro. Biochemistry. 31(36): 8654-60.
Hammarstrm P, et al. (2003) Prevention of transthyretin amyloid disease by changing protein misfolding energetics. Science. 299(5607): 713-6.

Human Transthyretin Proteins

Transthyretin Protein, Human, Recombinant (His Tag)12091-H07H

Mouse Transthyretin Proteins

Transthyretin Protein, Mouse, Recombinant (His Tag)50958-M08H

LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration
Author
Deng, M;Gui, X;Kim, J;Xie, L;Chen, W;Li, Z;He, L;Chen, Y;Chen, H;Luo, W;Lu, Z;Xie, J;Churchill, H;Xu, Y;Zhou, Z;Wu, G;Yu, C;John, S;Hirayasu, K;Nguyen, N;Liu, X;Huang, F;Li, L;Deng, H;Tang, H;Sadek, A;Zhang, L;Huang, T;Zou, Y;Chen, B;Zhu, H;Arase, H;Xia, N;Jiang, Y;Collins, R;You, M;Homsi, J;Unni, N;Lewis, C;Chen, G;Fu, Y;Liao, X;An, Z;Zheng, J;Zhang, N;Zhang, C;
Year
2018
Journal
Nature
PubMed ID: 30333625FoldedExpand

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Sino Biological/Transthyretin Protein, Human, Recombinant (His Tag)/1/12091-H08H

Description

Related Products

Citations

Protein Expression Service

Transthyretin Protein, Human, Recombinant (His Tag): Product Information

Transthyretin Protein, Human, Recombinant (His Tag): Images

Transthyretin Protein, Human, Recombinant (His Tag): Synonyms

Transthyretin Background Information

Human Transthyretin Proteins

Mouse Transthyretin Proteins

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