产品资料

Sino Biological/RSPO1 Protein, Mouse, Recombinant (His Tag)/1/50316-M08S

Description
Related Products
Citations
Protein Expression Service

RSPO1 Protein, Mouse, Recombinant (His Tag): Product Information

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">

Activity

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 0.1-0.9 μg/ml in the presence of 5 ng/mL recombinant mouse Wnt3a.

Protein Construction

A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_619624.2

Expressed Host

CHO Stable Cells

Species

Mouse

Predicted N Terminal

Ser 21

Molecule Mass

The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RSPO1 Protein, Mouse, Recombinant (His Tag): Images

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The _ED50 for this effect is typically 0.1-0.9 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.

RSPO1 Protein, Mouse, Recombinant (His Tag): Synonyms

R-spondin Protein, Mouse; Rspondin Protein, Mouse

RSPO1 Background Information

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Full Name

R-spondin 1

References

Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62.
Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.
Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

Human RSPO1 Proteins

RSPO1 Protein, Human, Recombinant (His Tag)11083-H08H
RSPO1 Protein, Human, Recombinant (aa 1-146, His Tag)11083-H08H1
RSPO1 Protein, Human, Recombinant11083-HNAS

Lgr4/Gpr48 negatively regulates TLR2/4-associated pattern recognition and innate immunity by targeting CD14 expression
Author
Du, B;Luo, W;Li, R;Tan, B;Han, H;Lu, X;Li, D;Qian, M;Zhang, D;Zhao, Y;Liu, M;
Year
2013
Journal
J. Biol. Chem.
Application
cell-based
PubMed ID: 23589304FoldedExpand
Fasting Activates Fatty Acid Oxidation to Enhance Intestinal Stem Cell Function during Homeostasis and Aging
Author
Mihaylova, MM;Cheng, CW;Cao, AQ;Tripathi, S;Mana, MD;Bauer-Rowe, KE;Abu-Remaileh, M;Clavain, L;Erdemir, A;Lewis, CA;Freinkman, E;Dickey, AS;La Spada, AR;Huang, Y;Bell, GW;Deshpande, V;Carmeliet, P;Katajisto, P;Sabatini, DM;Yilmaz, ÖH;
Year
2018
Journal
Cell Stem Cell
Application
cell-based
PubMed ID: 29727683FoldedExpand
Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet
Author
Cheng, CW;Biton, M;Haber, AL;Gunduz, N;Eng, G;Gaynor, LT;Tripathi, S;Calibasi-Kocal, G;Rickelt, S;Butty, VL;Moreno-Serrano, M;Iqbal, AM;Bauer-Rowe, KE;Imada, S;Ulutas, MS;Mylonas, C;Whary, MT;Levine, SS;Basbinar, Y;Hynes, RO;Mino-Kenudson, M;Deshpande, V;Boyer, LA;Fox, JG;Terranova, C;Rai, K;Piwnica-Worms, H;Mihaylova, MM;Regev, A;Yilmaz, ÖH;
Year
2019
Journal
Cell
Application
tissue culture
PubMed ID: 31442404FoldedExpand

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Sino Biological/RSPO1 Protein, Mouse, Recombinant (His Tag)/1/50316-M08S

Description

Related Products

Citations

Protein Expression Service

RSPO1 Protein, Mouse, Recombinant (His Tag): Product Information

RSPO1 Protein, Mouse, Recombinant (His Tag): Images

RSPO1 Protein, Mouse, Recombinant (His Tag): Synonyms

RSPO1 Background Information

Human RSPO1 Proteins

Citation List

If you couldn"t find the specific citation, you can:

E.coli protein expression service

Mammalian transient protein expression service

Baculovirus-insect expression service

Stable cell line development service (CHO / HEK293)