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AbD Serotec/IFN Gamma antibody | CC302/100 Tests/1ml/MCA1783A488

  • Summary
  • Secondary Antibodies
  • Negative Isotype Controls
  • External Images
  • References (48)
  • Spectraviewer
Mouse anti Bovine IFNγ antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000).Mouse anti bovine γ interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Mouse anti Bovine IFNγ antibody, clone CC302 has been used successfully for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using an ELISA.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Pig
Dog
Horse
Sheep
MustelidExpected from Sequence
Goat
Dolphin
Ferret
Mink
Fin Whale
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZEThis product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P07353
Entrez Gene
IFNG
GO Terms
GO:0009615response to virus
GO:0005125cytokine activity
GO:0005133interferon-gamma receptor binding
GO:0005615extracellular space
GO:0006955immune response
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry 11/201/200
Flow Cytometry 11/101/100
ELISA5ug/ml
Flow Cytometry 11/10
ELISA
Flow Cytometry 11/1001/500
Flow Cytometry 1Neat1/10
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
    1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
    1. 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
    1. 1 Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.For use on Equine samples Bio-Rad recommend MCA1783F
    Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    Flow Cytometry
    Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    ELISA
    Biotinylated mouse anti bovine IFNγ, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFNγ, clone CC330, as the capture reagent and recombinant bovine IFNγ as the standard.
    ELISA
    Biotinylated mouse anti bovine IFNγ, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFNγ, clone CC330, as the capture reagent and recombinant bovine IFNγ as the standard.
    Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

    Secondary Antibodies Available

    DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
    Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE WB 0.5 mgloader
    Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF 0.5 mgloader
    Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117PE WB 0.5 mgloader
    Goat anti Mouse IgG (Fc):FITCSTAR120FC F 1 mgloader
    Goat anti Mouse IgG (Fc):HRPSTAR120PE WB 1 mgloader
    Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR70F 0.5 mgloader
    Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR77C E P 0.5 mgloader
    Goat anti Mouse IgG/A/M:Alk. Phos.STAR87AC E WB 1 mgloader
    Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87PE 1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GAF IF WB 0.1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9BF 1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13BC E P RE WB 1 mgloader
    Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GAF IF WB 0.1 mgloader
    Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GAFIF 0.1 mgloader
    Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GAF WB 0.1 mgloader
    Rabbit F(ab')2 anti Mouse IgG:RPESTAR12AF 1 mlloader
    Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR76F 1 mlloader

    Negative Isotype Controls Available

    DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
    Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488F 100 Tests/1mlloader
    Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647F 100 Tests/1mlloader
    Mouse IgG1 Negative Control:FITCMCA928FF 100 Testsloader
    Mouse IgG1 Negative ControlMCA928F 100 Testsloader
    Mouse IgG1 Negative Control:RPEMCA928PEF 100 Testsloader

    Application Based External Images

    ELISA

    Flow Cytometry

    Product Specific References

    References for IFN Gamma antibody

    1. Hasvold, H.J. et al. (2002) In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium avium subsp paratuberculosis.Vet Immunol Immunopathol. 90 (1-2): 79-89.
    2. Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.J Immunol. 169 (7): 3837-46.
    3. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.Vet Immunol Immunopathol. 88 (3-4): 111-22.
    4. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.Clin Diagn Lab Immunol. 9 (6): 1229-34.
    5. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host.J Gen Virol. 90: 2157-65.
    6. Jaber, J.R. et al. (2010) Cross-reactivity of anti-human, anti-porcine and anti-bovine cytokine antibodies with cetacean tissues.J Comp Pathol. 143: 45-51.
    7. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15.
    8. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
    9. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19.
    10. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.Clin Vaccine Immunol. 16 (7): 1003-11.
    11. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.J Immunol. 185: 3313-25.
    12. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.Vet Immunol Immunopathol. 140: 175-80.
    13. Pillet, S. et al. (2011) Cellular immune response in the presence of protective antibody levels correlates with protection against 1918 influenza in ferrets.Vaccine. 29 (39): 6793-801.
    14. Jensen, T.H. et al. (2009) Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.Vaccine. 27: 5178-83.
    15. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.PLoS One. 6:e21978.
    16. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS One. 6: e29194.
    17. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92.
    18. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.Vet Immunol Immunopathol. 146: 1-7.
    19. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.Pol J Vet Sci. 15 (1): 11-20.
    20. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.Clin Vaccine Immunol. 20: 1669-74.
    21. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 2014 Dec 16;10(1):293.
    22. Köhler. H, et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection BMC Veterinary Research. 11 (1): 74.
    23. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.Vet Parasitol. 220: 33-45.
    24. Rodríguez-Gómez IM et al. (2016) Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells.Dev Comp Immunol. 60: 115-26.
    25. Taylor, G. et al. (2015) Efficacy of a virus-vectored vaccine against human and bovine respiratory syncytial virus infections.Sci Transl Med. 7 (300): 300ra127.
    26. Moreira, M.L. et al. (2015) Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining.Acta Vet Scand. 57: 51.
    27. El-Naggar, M.M. et al. (2015) Development of an improved ESAT-6 and CFP-10 peptide-based cytokine flow cytometric assay for bovine tuberculosis.Comp Immunol Microbiol Infect Dis. 42: 1-7.
    28. McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.PLoS One. 11 (2): e0148229.
    29. Vida, B. et al. (2016) Immunologic progression of canine leishmaniosis following vertical transmission in United States dogs.Vet Immunol Immunopathol. 169: 34-8.
    30. Totté, P. et al. (2010) CD62L defines a subset of pathogen-specific bovine CD4 with central memory cell characteristics.Dev Comp Immunol. 34 (2): 177-82.
    31. Sun, L. et al. (2012) The role of proliferation in the regulation of interferon gamma (IFNγ) expression in foals.Dev Comp Immunol. 36 (3): 534-9.
    32. Reber, A.J. et al. (2006) Evaluation of multiple immune parameters after vaccination with modified live or killed bovine viral diarrhea virus vaccines.Comp Immunol Microbiol Infect Dis. 29 (1): 61-77.
    33. Katepalli, M.P. et al. (2008) The effect of age and telomere length on immune function in the horse.Dev Comp Immunol. 32 (12): 1409-15.
    34. Hansen, S. et al. (2013) Age-related changes in intracellular expression of IFN-γ and TNF-α in equine lymphocytes measured in bronchoalveolar lavage and peripheral blood.Dev Comp Immunol. 39 (3): 228-33.
    35. Boshra H et al. (2015) A lumpy skin disease virus deficient of an IL-10 gene homologue provides protective immunity against virulent capripoxvirus challenge in sheep and goats.Antiviral Res. 123: 39-49.
    36. Hedges, J.F. et al. (2015) Amphotericin B stimulates γδ T and NK cells, and enhances protection from Salmonella infection.Innate Immun. 21 (6): 598-608.
    37. Johnson, W.C. et al. (2008) Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18. Dev Comp Immunol. 32 (8): 1002-10.
    38. Dewals, B.G., et al.l (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissuesVet Res. 42(1): 95.
    39. Maggioli, M.F. et al. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.Front Immunol. 7: 421.
    40. Cassady-cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
    41. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.Vet Res. 48 (1): 20.
    42. DaSilva, A.V.A. et al. (2018) Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens.PLoS Negl Trop Dis. 12 (4): e0006445.
    43. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.Vet Immunol Immunopathol. 202: 74-84.
    44. Roos, E.O. et al. (2018) IP-10: A potential biomarker for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus).Vet Immunol Immunopathol. 201: 43-8.
    45. Aguiar-Soares, R.D.O. et al. (2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune®, and Leish-Tec® Vaccines against Canine Visceral Leishmaniasis.Vaccines (Basel). 8 (4)Nov 17 [Epub ahead of print].
    46. Fedorka, C.E. et al. (2019) Alteration of the mare"s immune system by the synthetic progestin, altrenogest.Am J Reprod Immunol. 82 (2): e13145.
    47. Lacasta, A. et al. (2021) Synergistic Effect of Two Nanotechnologies Enhances the Protective Capacity of the Theileria parva Sporozoite p67C Antigen in Cattle.J Immunol. Jan 08 [Epub ahead of print].

    Further Reading

    1. Rhodes, S. et al. (2000) Distinct response kinetics of gamma interferon and interleukin-4 in bovine tuberculosis.Infect Immun. 68:5393-400.

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    CiteAb logo - trusted, tested, published

    Our IFN Gamma (CC302) Antibody has been referenced in >83 publications*

    *Based on June 2020 data from CiteAb"s antibody search engine.

    View more products with IFN GAMMA specificity
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