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AbD Serotec/IL-4 antibody | CC314/0.25 mg/MCA2372B

Summary
External Images
References (7)
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Mouse anti Bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Mouse anti Bovine IL-4 antibody, clone CC313 (MCA2371) as a coating reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Recombinant bovine IL-4.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells form immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P30367
Entrez Gene: IL4
GO Terms: GO:0005125cytokine activity; GO:0005136interleukin-4 receptor binding; GO:0005615extracellular space; GO:0006955immune response; GO:0008083growth factor activity; GO:0030890positive regulation of B cell proliferation; GO:0042113B cell activation; GO:0043066negative regulation of apoptosis; GO:0045348positive regulation of MHC class II biosynthetic process; GO:0045671negative regulation of osteoclast differentiation; GO:0045944positive regulation of transcription from RNA polymerase II promoter; GO:0048295positive regulation of isotype switching to IgE isotypes; GO:0048304positive regulation of isotype switching to IgG isotypes
Regulatory: For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1ug/ml	5ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
ELISA: This biotin conjugate may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with MCA2371 as capture reagent, and recombinant bovine IL-4 (PBP006) as a standard.

Application Based External Images

ELISA

Product Specific References

References for IL-4 antibody

Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.J Immunol Methods. 301 (1-2): 114-23.
Abbott JR et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.J Immunol. 174 (11): 6702-15.
Stabel JR et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.Clin Vaccine Immunol. 20 (6): 848-57.
Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.Clin Vaccine Immunol. 18: 2079-89.
Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.Res Vet Sci. 93: 662-7.
Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.PLoS One. 7: e30430.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]

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AbD Serotec/IL-4 antibody | CC314/0.25 mg/MCA2372B

Product Details

Storage Information

More Information

Applications of IL-4 antibody

Application Based External Images

ELISA

Product Specific References

References for IL-4 antibody

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