产品资料

AbD Serotec/IL-6 antibody/50 µg/AHP2380B

Summary
Spectraviewer

Chicken anti Bovine interleukin-6 antibody recognizes bovine interleukin-6 (IL-6), a 23.8 kDa cytokine belonging to the IL-6 superfamily.Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the acute phase response and also as an anti-inflammatory myokine (Febbraio & Pedersen 2005).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep	Weak
Cat
Dog
Horse
Human
Mouse
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to bovine interleukin-6 were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Recombinant bovine interleukin-6

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.This product should be stored undiluted.
Storage: Store at +4^oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

UniProt: P26892
Entrez Gene: IL6
GO Terms: GO:0006953acute-phase response; GO:0005125cytokine activity; GO:0005615extracellular space; GO:0006955immune response; GO:0008083growth factor activity
Regulatory: For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.1ug/ml	0.5ug/ml
ELISA	1ug/ml	5ug/ml
Western Blotting	0.1ug/ml	2ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This product may be used as a detection antibody in a sandwich ELISA together with AHP2380 as the capture reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
ELISA: This product may be used as a capture antibody in a sandwich ELISA together with AHP2380B as the detection reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.

Fluorescent Spectraviewer

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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

View more products with IL-6 specificity

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AbD Serotec/IL-6 antibody/50 µg/AHP2380B

Product Details

Storage Information

More Information

Applications of IL-6 antibody

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸