产品资料

AbD Serotec/IL-10 antibody | CC318/0.5 mg/MCA2110

Mouse anti Bovine Interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Horse
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Plasmid cDNA encoding bovine IL-10.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P43480
Entrez Gene: IL10
GO Terms: GO:0002237response to molecule of bacterial origin; GO:0002740negative regulation of cytokine secretion involved in immune response; GO:0002904positive regulation of B cell apoptosis; GO:0005125cytokine activity; GO:0005615extracellular space; GO:0006954inflammatory response; GO:0006955immune response; GO:0030889negative regulation of B cell proliferation; GO:0032715negative regulation of interleukin-6 production; GO:0032800receptor biosynthetic process; GO:0042095interferon-gamma biosynthetic process; GO:0043193positive regulation of gene-specific transcription; GO:0045019negative regulation of nitric oxide biosynthetic process; GO:0045077negative regulation of interferon-gamma biosynthetic process; GO:0050715positive regulation of cytokine secretion; GO:0051045negative regulation of membrane protein ectodomain proteolysis; GO:0051091positive regulation of transcription factor activity; GO:0051384response to glucocorticoid stimulus
Regulatory: For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	5ug/ml	10ug/ml
ELISpot
Flow Cytometry ¹

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a capture antibody in a sandwich ELISA assay for bovine IL-10 in combination with MCA2111B as detection reagent, see Bannerman, D.D.et al.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG2b Negative Control	MCA691	F	100 Tests	Log in		Get Quote

Application Based External Images

ELISA

ELISpot

Flow Cytometry

Product Specific References

References for IL-10 antibody

Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.Vet Immunol Immunopathol. 85 (3-4): 213-23.
Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.Infect Immun. 78: 1383-9.
Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.J Leukoc Biol. 83 (1): 48-55.
Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.Int Arch Allergy Immunol. 144: 325-37.
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70.
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.Funct Integr Genomics. 10: 21-38.
Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.Clin Experiment Ophthalmol. 38: 405-13.
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19.
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72.
Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.Vet Res. 41: 14.
den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.PLoSone 6: e18188
Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.Vet Res. 44: 59.
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705.
Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.Clin Vaccine Immunol. 17: 1946-51.
McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.Immunology. 139: 227-44.
Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.PLoS One. 7: e30430.
Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne"s disease.Clin Vaccine Immunol. 18: 1467-76.
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.PLoS One. 10 (5): e0124144.
Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.PLoS One. 11 (4): e0154172.
Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection. Res Vet Sci. 110: 34-39.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
Pomeroy B et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.Vet Immunol Immunopathol. 182: 37-42.
Davidson, J.O. et al. (2020) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.Stem Cells Transl Med. Oct 26 [Epub ahead of print].

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AbD Serotec/IL-10 antibody | CC318/0.5 mg/MCA2110

Product Details

Storage Information

More Information

Applications of IL-10 antibody

Secondary Antibodies Available

Negative Isotype Controls Available

Application Based External Images

ELISA

ELISpot

Flow Cytometry

Product Specific References

References for IL-10 antibody

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸