产品资料

AbD Serotec/IL-10 antibody | CC320/0.25 mg/MCA2111B

Mouse anti Bovine Interleukin-10 antibody, clone CC320 recognizes bovine IL-10.Mouse anti Bovine Interleukin-10 antibody, clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Horse
Sheep
Goat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

None present

Carrier Free

Yes

Immunogen

Plasmid cDNA encoding bovine IL-10.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted.Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only. This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

UniProt: P43480
Entrez Gene: IL10
GO Terms: GO:0002237response to molecule of bacterial origin; GO:0002740negative regulation of cytokine secretion involved in immune response; GO:0002904positive regulation of B cell apoptosis; GO:0005125cytokine activity; GO:0005615extracellular space; GO:0006954inflammatory response; GO:0006955immune response; GO:0030889negative regulation of B cell proliferation; GO:0032715negative regulation of interleukin-6 production; GO:0032800receptor biosynthetic process; GO:0042095interferon-gamma biosynthetic process; GO:0043193positive regulation of gene-specific transcription; GO:0045019negative regulation of nitric oxide biosynthetic process; GO:0045077negative regulation of interferon-gamma biosynthetic process; GO:0050715positive regulation of cytokine secretion; GO:0051045negative regulation of membrane protein ectodomain proteolysis; GO:0051091positive regulation of transcription factor activity; GO:0051384response to glucocorticoid stimulus
Regulatory: For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	2ug/ml	5ug/ml
ELISA	5ug/ml	10ug/ml
Flow Cytometry ¹
Functional Assays

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This biotin conjugate may be used as detection reagent in a sandwich ELISA assay for bovine IL-10 with MCA2110 as capture reagent, see Bannerman, D.D.et al.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control:Low Endotoxin	MCA928EL	C F	0.5 mg	Log in		Get Quote

Application Based External Images

ELISA

ELISpot

Flow Cytometry

Functional Assays

Product Specific References

References for IL-10 antibody

Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.Vet Immunol Immunopathol. 85 (3-4): 213-23.
Buza JJ et al. (2004) Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis.Infect Immun. 72 (4): 2425-8.
Scandurra, G.M. et al. (2010) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.Infect Immun. 78 (3): 1383-9.
Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.J Leukoc Biol. 83 (1): 48-55.
Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.Int Arch Allergy Immunol. 144: 325-37.
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70.
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.Funct Integr Genomics. 10: 21-38.
Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.Clin Experiment Ophthalmol. 38: 405-13.
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19.
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.Clin Vaccine Immunol. 16 (7): 1003-11.
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705.
Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.J Virol. 82: 1777-86.
Ma&347;lanka T et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.Pol J Vet Sci. 15 (1): 11-20.
Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.J Comp Pathol. 148: 157-72.
Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.PLoS One. 10 (5): e0124144.
Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.PLoS One. 11 (4): e0154172.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
Pomeroy, B. et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.Vet Immunol Immunopathol. 182: 37-42.
Pomeroy, B. et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.Vet Immunol Immunopathol. 167 (1-2): 22-9.
Davidson, J.O. et al. (2020) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.Stem Cells Transl Med. Oct 26 [Epub ahead of print].
Borghesi, J. et al. (2020) Effects of doxorubicin associated with amniotic membrane stem cells in the treatment of canine inflammatory breast carcinoma (IPC-366) cells.BMC Vet Res. 16 (1): 353.

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AbD Serotec/IL-10 antibody | CC320/0.25 mg/MCA2111B

Product Details

Storage Information

More Information

Applications of IL-10 antibody

Secondary Antibodies Available

Negative Isotype Controls Available

Application Based External Images

ELISA

ELISpot

Flow Cytometry

Functional Assays

Product Specific References

References for IL-10 antibody

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸