产品资料

Addgene/pAAV-hSyn-hM3D(Gq)-mCherry/1unit/50474-AAV2

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	50474	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV2	50474-AAV2	Virus (100 µL at titer ≥ 3×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV5	50474-AAV5	Virus (100 µL at titer ≥ 3×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV8	50474-AAV8	Virus (100 µL at titer ≥ 2×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV Retrograde	50474-AAVrg	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pAAV
(Search Vector Database)
Backbone sizew/o insert(bp)4818
Total vector size (bp)7070
Vector type
AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
Low Copy

Gene/Insert

Gene/Insert name
hM3D(Gq)-mCherry
Species
H. sapiens (human)
Insert Size (bp)
2502
Mutation
See supplemental documents for DREADD mutations
Entrez Gene
CHRM3(a.k.a. EGBRS, HM3, PBS)
Promoterhuman Synapsin 1
Tag/ Fusion Protein
- mCherry (C terminal on insert)

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteSal I(not destroyed)
3′ cloning siteEcoR I(not destroyed)
5′ sequencing primerTCGTGTCGTGCCTGAGAGCG
3′ sequencing primerGCATTAAAGCAGCGTATCCACATAGC

Resource Information

Supplemental Documents
- DREADD mutations
Terms and Licenses
- UBMTA
- Takara Bio Limited Use Label License (formerly Clontech)
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 5 References

Depositor Comments

Please Note- This plasmid does NOT contain an N-terminal HA tag.

For more information on DREADDs, see: http://pdspit3.mml.unc.edu/projects/dreadd/wiki/WikiStart

Information for AAV2 (Catalog # 50474-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA.

hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 3×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV2
PurificationCsCl gradient ultracentrifugation
Reporter GenemCherry

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Data submitted about 50474-AAV2 by requesting scientist(s):

Data 1: Rat, Brain parenchyma

Information for AAV5 (Catalog # 50474-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA.

hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 3×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GenemCherry

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 50474-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA.

hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 2×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GenemCherry

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV Retrograde (Catalog # 50474-AAVrg)(Back to top)

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA.

hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GenemCherry

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Data submitted about 50474-AAVrg by requesting scientist(s):

Data 1: Rat, Brain parenchyma

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Addgene/pAAV-hSyn-hM3D(Gq)-mCherry/1unit/50474-AAV2

Ordering

Backbone

Growth in Bacteria

Gene/Insert

Cloning Information

Resource Information

Depositor Comments

Information for AAV2 (Catalog # 50474-AAV2)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control

Addgene Comments

Information for AAV5 (Catalog # 50474-AAV5)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control

Information for AAV8 (Catalog # 50474-AAV8)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control

Information for AAV Retrograde (Catalog # 50474-AAVrg)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control

Addgene Comments