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Addgene/pLentiN 8E6/1unit/37450

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ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid37450Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti 6.3/V5 DEST
    (Search Vector Database)
  • Backbone manufacturer
    Invitrogen
  • Backbone sizew/o insert(bp)9387
  • Total vector size (bp)8623
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 8E6
  • Species
    HPV
  • Insert Size (bp)
    542
  • PromoterCMV
  • Tags/ Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning methodGateway Cloning
  • 5′ sequencing primerCMV forward
  • 3′ sequencing primerV5 reverse
    • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The 8E6 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)

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