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Addgene/155_KRAS in pmiRGlo/1unit/78132

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ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid78132Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmiRGlo
    (Search Vector Database)
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KRAS
  • Species
    H. sapiens (human)
  • Mutation
    3'UTR containing miRNA binding sites
  • Entrez Gene
    KRAS(a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • PromoterPGK
  • Tag/ Fusion Protein
    • luciferase (N terminal on backbone)

Cloning Information

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteNheI(not destroyed)
  • 3′ cloning siteXbaI(not destroyed)
  • 5′ sequencing primerFluc-F1 (AGAAGCTGCGCGGTGGTGTTGTG)
  • 3′ sequencing primerT7terminal
    • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
    • Luciferase Limited Use Label License
  • Industry Terms
    • Not Available to Industry
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