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Millipore/MAB1397Z | Anti-ICAM-1 Antibody, clone 3E2B, Azide Free/MAB1397Z/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Millipore/MAB1397Z | Anti-ICAM-1 Antibody, clone 3E2B, Azide Free/MAB1397Z/100 µg

Description
CatalogueNumberMAB1397Z
ReplacesCBL1331F
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-ICAM-1Antibody,clone3E2B,AzideFree
AlternateNames
  • CD54
  • MAB1397
ProductInformation
FormatPurified
PresentationPurifiedIgGpreparedbyaffinitychromatographyonProteinGagarosebeads.Liquidat1mg/mLinsterile-filteredPBS,containingnopreservatives.Endotoxinlevel4.13EU/mg
StorageandShippingInformation
StorageConditionsMaintainfrozenat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze-thawcycles.
Applications
ApplicationAnti-ICAM-1Antibody,clone3E2B,AzideFreedetectslevelofICAM-1&hasbeenpublished&validatedforuseinELISA,FC,FUNC,IC,IH.
KeyApplications
  • ELISA
  • FlowCytometry
  • AffectsFunction
  • Immunocytochemistry
  • Immunohistochemistry
ApplicationNotesImmunocytochemistry:Liveoracetonefixedcells

ELISA

Immunohistochemistryofacetonefixedfrozensections.(Paraffinnottested).

FlowCytometry:Suggestedworkingdilution50μg/mL.Use5mLofthisdilutiontolabel0.5x10(e6)cells.

Immunoprecipitation:useproteinGorrabbitanti-hamsterIgGsecondary

Clone3E2BisreportedtobefunctionblockingagainstmurineICAM-1.

Optimalworkingdilutionsmustbedeterminedbyenduser.
BIOLOGicalInformation
Clone3E2B
HostArmenianHamster
SpecificityMAB1371ZisreactivewithmouseICAM-1(CD54),whichispresentonT-cells,B-cells,monocytesandendothelialcells,epithelialcells,dendriticcellsandkeratinocytes.ExpressionofCD54isincreasedafterstimulationwithcytokinesandLPS,anditalsoplaysaroleinBcell-Tcellinteractions.
IsotypeIgG
SpeciesReactivity
  • Mouse
AntibodyTypeMonoclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryICAM1(CD54)istypicallyexpressedonendothelialcellsandcellsoftheimmunesystem.ICAM1bindstointegrinsoftypeCD11a/CD18,orCD11b/CD18.ICAM1isalsoexploitedbyRhinovirusasareceptor.
GeneSymbol
  • ICAM1
  • P3.58
  • BB2
  • CD54
  • ICAM-1
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P05362#ICAMproteinsareligandsfortheleukocyteadhesionproteinLFA-1(integrinalpha-L/beta-2).Incaseofrhinovirusinfectionactsasacellularreceptorforthevirus.Alsoactsasreceptorforsomecoxsackieviruses.
SIZE:532aminoacids;57825Da
SUBUNIT:Interactswithhumanherpesvirus8MIR2protein(Probable).
SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
PTM:Monoubiquitinated,whichispromotedbyMARCH9andleadstoendocytosis.
SIMILARITY:SwissProt:P05362##Belongstotheimmunoglobulinsuperfamily.ICAMfamily.&Contains5Ig-likeC2-type(immunoglobulin-like)domains.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
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