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Millipore/AB3830 | Anti-Caveolin-1 Antibody, phospho-specific (Tyr14)/AB3830/100 µL188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Millipore/AB3830 | Anti-Caveolin-1 Antibody, phospho-specific (Tyr14)/AB3830/100 µL

Description
CatalogueNumberAB3830
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-Caveolin-1Antibody,phospho-specific(Tyr14)
ProductInformation
FormatAffinityPurified
PresentationProteinAandpeptideaffinitypurifiedimmunoglobulin.Liquidin10mMsodiumHEPES,pH7.5,150mMNaCl,100μg/mlBSA,with50%glycerol.
StorageandShippingInformation
StorageConditionsMaintainat-20ºCfor12months,donotaliquot.
Applications
ApplicationThisAnti-Caveolin-1Antibody,phospho-specific(Tyr14)isvalidatedforuseinIP,WBforthedetectionofCaveolin-1.
KeyApplications
  • Immunoprecipitation
  • WesternBlotting
ApplicationNotesWesternblotting1:1,000(forbestresults,incubatemembranewithdilutedantibodyin5%BSA,1XTBS,0.1%Tween-20at4°Cwithgentleshaking,overnight)

Immunoprecipitation1:50

Optimalworkingdilutionsmustbedeterminedbytheenduser.
BIOLOGicalInformation
ImmunogenKLH-conjugated,syntheticphospho-peptidecorrespondingtoresiduessurroundingTyr14ofhumancaveolin-1
Epitopephospho-specific(Tyr14)
HostRabbit
SpecificityRecognizescaveolin-1onlywhenphosphorylatedattyrosine14.Thisantibodydoesnotcross-reactwithcaveolin-2,-3orcaveolin-1beta,ortheshortisoformofcaveolin-1.
SpeciesReactivity
  • Human
  • Mouse
  • Monkey
  • Rat
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryThescaffoldingproteinencodedbythisgeneisthemaincomponentofthecaveolaeplasmamembranesfoundinmostcelltypes.TheproteinlinksintegrinsubunitstothetyrosinekinaseFYN,aninitiatingstepincouplingintegrinstotheRas-ERKpathwayandpromotingcellcycleprogression.ThegeneisatumorsuppressorgenecandidateandanegativeregulatoroftheRas-p42/44MAPkinasecascade.CAV1andCAV2arelocatednexttoeachotheronchromosome7andexpresscolocalizingproteinsthatformastablehetero-oligomericcomplex.ByusingalternativeinitiationcodonsinthesamereADIngframe,twoisoforms(alphaandbeta)areencodedbyasingletranscriptfromthisgene.
GeneSymbol
  • CAV1
  • MSTP085
  • CAV
  • VIP21
  • Caveolin-1.
Modifications
  • Phosphorylation
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:Q03135#Mayactasascaffoldingproteinwithincaveolarmembranes.InteractsdirectlywithG-proteinalphasubunitsandcanfunctionallyregulatetheiractivity(Bysimilarity).
SIZE:178aminoacids;20472Da
SUBUNIT:Homooligomer.InteractswithGLIPR2,NOSTRIN,SNAP25andsyntaxin.
SUBCELLULARLOCATION:Golgiapparatusmembrane;Peripheralmembraneprotein;Cytoplasmicside.Cellmembrane,caveola;Peripheralmembraneprotein;Cytoplasmicside.Note=Membraneproteinofcaveolae.Potentialhairpin-likestructureinthemembrane.
TISSUESPECIFICITY:Inmuscleandlung,lesssoinliver,brainandkidney.
PTM:TheinitiatormethionineforisoformBetaisremovedduringorjustaftertranslation.ThenewN-terminalaminoacidisthenN-acetylated.
SIMILARITY:SwissProt:Q03135##Belongstothecaveolinfamily.
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
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