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| Description | |
|---|---|
| CatalogueNumber | 04-282 |
| BrandFamily | Upstate |
| TradeName |
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| Description | Anti-phospho-EGFR(Thr654)Antibody,clone3F2 |
| AlternateNames |
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| ProductInformation | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | 100µg(0.1mg/ml)ofmousemonoclonalIgG1inPBScontaining0.09%sodiumazide,PEG,sucroseand50%glycerol |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | 2yearsat-20°Cfromdateofshipment |
| Applications | |
|---|---|
| Application | Detectphospho-EGFR(Thr654)usingthisAnti-phospho-EGFR(Thr654)Antibody,clone3F2validatedforuseinELISA,IP&WB. |
| KeyApplications |
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| BIOLOGicalInformation | |
|---|---|
| Immunogen | KLH-conjugatedsyntheticpeptideencompassingthesurroundingaminoacidsofThr654inhumanEGFR |
| Clone | 3F2 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | RecognizesphosphorylatedEGFRonThreonine654 |
| Isotype | IgG1 |
| SpeciesReactivity |
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| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | Subsequentthiophilicadsorptionandsizeexclusionchromatography |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. |
| MolecularWeight | 180kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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