Description | |
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CatalogueNumber | 16-251 |
BrandFamily | Upstate |
TradeName |
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Description | Anti-phospho-HistoneH2B(Ser14)Antibody,cloneMC603,FITCconjugate,rabbitmonoclonal |
AlternateNames |
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ProductInformation | |
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Format | FITC |
StorageandShippingInformation | |
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StorageConditions | 2yearsat4°Cfromdateofshipment |
BIOLOGicalInformation | |
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Immunogen | KLH-conjugatedsyntheticpeptidecontainingthesequenceKGpSKinwhichpScorrespondstophospho-serine14ofhumanHistoneH2B.CloneMC603. |
Clone | MC603 |
Host | Rabbit |
Specificity | phospho-histoneH2B(Ser14) |
Isotype | IgG |
SpeciesReactivity |
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SpeciesReactivityNote | Broadspeciescross-reactivityexpectedbasedonsequencehomology. |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement. |
GeneSymbol |
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Modifications |
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PurificationMethod | ProteinAPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:126aminoacids;13908Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination. SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily. |
MolecularWeight | 15kDa |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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