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| Description | |
|---|---|
| CatalogueNumber | 07-819 |
| BrandFamily | Upstate |
| TradeName |
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| Description | Anti-phospho-EGFR(Tyr1148)Antibody |
| ProductInformation | |
|---|---|
| Format | AffinityPurified |
| Presentation | 100μlofaffinitypurifiedrabbitpolyclonalIgGin50%storagebuffer(PBS(withoutMg2+andCa2+),pH7.3containing1.0mg/mLBSA(IgG,proteasefree)and0.05%sodiumazide)and50%glycerol. |
| StorageandShippingInformation | |
|---|---|
| StorageConditions | Stablefor2yearsat-20°Cfromdateofshipment. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BIOLOGicalInformation | |
|---|---|
| Immunogen | Peptidecorrespondingtoaminoacidregionencompassingthehuman,mouse,andratphospho-EGFR(Tyr1148) |
| Host | Rabbit |
| Specificity | Recognizesphospho-EGFR(Tyr1148) |
| SpeciesReactivity |
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| SpeciesReactivityNote | Predictedtocross-reactwithmouseandratbasedonsequencehomology(100%),buthavenotbeentested. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| GeneSymbol |
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| Modifications |
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| PurificationMethod | ImmunoAffinityPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. |
| MolecularWeight | 185kDa |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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