merck millipore,默克密理博,17295,Chromatin Immunoprecipitation (ChIP) Assay Kit

merckmillipore,默克密理博,17-295,ChromatinImmunoprecipitation(ChIP)AssayKit产品名称：ChromatinImmunoprecipitation(ChIP)AssayKit
产品型号：17-295
Containsallnecessaryreagentstoperform22individualchromatinimmunoprecipitation(ChIP)reactionsusinginexpensiveproteinAagarosebeads.

merckmillipore,默克密理博,17-295,ChromatinImmunoprecipitation(ChIP)AssayKit
Forusetoenrichforspecificregionsofchromatinthatareimmunoprecipitatedwithaspecificantibodyforachromatinprotein.Workswithalleukaryoticcelltypes.Detectionofthegeneorpromoterofinterestinimmunoprecipitatedchromatinmustbeempiricallydeterminedbytheresearcher.QuantitativePCRdetectionisrecommended.
ChromatinImmunoprecipitation(ChIP)isapowerfultechniqueformappingtheinvivodistributionofproteinsassociatedwithchromosomalDNA.Theseproteinscanbehistonesubunitsandpost-translationalmodificationsorotherchromatinassociatedproteinssuchastranscriptionfactors,chromatinregulators,etc.Additionally,ChIPcanbeusedtoidentifyregionsofthegenomeassociatedwiththeseproteins,orconversely,toidentifyproteinsassociatedwithaparticularregionofthegenome.ChIPmethodologyofteninvolvesprotein-DNAandprotein-proteincross-linking,fragmentationofthecross-linkedchromatin,andsubsequentimmunoprecipitationofchromatinwithanantibodyspecifictoatargetprotein.TheDNAfragmentsisolatedincomplexwiththetargetproteincanbeidentifiedbyavarietyofmethodsincludingPCR,DNAmicroarrayandDNAsequencing.StandardorquantitativePCRcanbeperformedtoverifywhetheraparticularDNAsequence(thegeneorregionofthegenome)isassociatedwiththeproteinofinterest.ThecombinationofChIPandpromoterorgenomictilingmicroarrays(ChIP-chip)allowsgenome-wideidentificationofDNA-bindingsitesforchromatin-associatedproteinswithpreciseresolution.Alternatively,high-throughputsequencingoflibrariesconstructedfromimmunoprecipitatedchromosomalDNA(ChIP-Seq)isapowerfulalternativetoChIP-chipinmappingtheprotein-DNAinteractionsacrossmammaliangenomes.
Containsallnecessaryreagentstoperform22individualchromatinimmunoprecipitation(ChIP)reactions.Suppliedbuffersaresufficienttogeneratechromatinfromuptofive15cmplatesofculturedcells,eachplateprovidingupto10chromatinpreparations(varieswithcellandassaytype).
Containsallnecessaryreagentstoperform22individualchromatinimmunoprecipitation(ChIP)reactionsusinginexpensiveproteinAagarosebeads.
Routinelyevaluatedusinganti-acetylHistoneH4polyclonalantibody(06-866)toimmunoprecipitateacetylatedhistoneH4.Subsequentimmunoblotanalysiswasdoneusingthesameantibody.
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