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| Description | |
|---|---|
| CatalogueNumber | 17-10246 |
| TradeName |
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| Description | ChIPAb+™HistoneH2A.BBD-ChIPValidatedAntibodyandPrimerSet |
| Overview | AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2A.BbdsetincludestheHistoneH2A.Bbdantibody,aNormalRabbitIgG,andcontrolprimerswhichamplifya70bpregionofChIPprimers,PGK1.TheHistoneH2A.Bbdandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Bbd-associatedchromatin. |
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| BackgroundInformation | HistoneH2A.Bbd(Barrbody-deficient),alsoknownashistoneH2A-Bbdtype1,isanatypicalhistonevariant.ThishistonevariantassociateswithH2BandreplaceshistoneH2Ainthenucleosome.IncontrasttootherH2Ahistones,thisvariantlacksconservedresiduesthataretargetsofpost-translationalmodifications.ConsequentlythisH2A.Bbdvariantisabout15aminoacidsshorterandonly48%similartothecanonicalH2A.ThemajordifferencesbetweenthetwoarefoundinthedockingdomainthatlinksH2A-H2BtotheH3-H4tetramer.Nucleosomescontainingthishistoneadoptamorerelaxedstructureandprotect~118basepairsofDNAinsteadofthe147basepairsfoundinclassicalnucleosomes.ThisvarianthistoneisassociatedwithactiveXchromosomesandautosomes,andistypicallyabsentfrominactiveXchromosomes. |
| ProductInformation | |
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| Format | AffinityPurified |
| Control |
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| Presentation | Anti-HistoneH2A.Bbd(rabbitpolyclonal).Onevialcontaining100µgofpurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforetheadditionof30%glycerol.Storeat-20°C Concentration:0.7mg/mL NormalRabbitIgG,.Onevialcontaining125µgofRabbitIgGin125µLofstoragebuffercontaining0.05%sodiumazide.Storeat-20°C. ChIPprimers,PGK1.Onevialcontaining75μLof5μMofeachprimerspecificforhumanPGK1.Storeat-20°C. FOR:CCTTCCCCCACGCTCCTA REV:TTGTGCAGGAACCTGTGAGATG |
| StorageandShippingInformation | |
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| StorageConditions | Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance. Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage. |
| BiologicalInformation | |
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| Immunogen | KLH-conjugatedlinearpeptidecorrespondingtohumanHistoneH2A.Bbd. |
| Epitope | a.a.35-55 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisantibodyrecognizeshumanHistoneH2A.Bbd. |
| SpeciesReactivity |
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| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.ThisgeneispartofaregionthatisrepeatedthreetimesonchromosomeX,onceinintron22oftheF8geneandtwiceclosertotheXqtelomere.Thisrecordrepresentsthemostcentromericcopywhichisinintron22oftheF8gene.[providedbyRefSeq]. |
| GeneSymbol |
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| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:AtypicalhistoneH2AwhichcanreplaceconventionalH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Nucleosomescontainingthishistonearelessrigidandorganizeonly118basepairofDNAinsteadof147inclassicalnucleosomes.TheyareassociatedwithtranscriptionallyactivechromatinandexcludedformBarrbodies.Ref.4Ref.5 SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Maybeincorporatedintoaproportionofnucleosomes,replacingoneormoreH2Amolecules. SUBCELLULARLOCATION:Nucleus.Chromosome.Note:AssociatedwiththeactiveXchromosomeandwithautosomes,whileitisabsentfromtheinactiveXchromosomeandexcludedfromBarrbodies. DOMAIN:ThedockingdomainisresponsiblefortheweakerheterodimerizationwithH2B.Ref.4 MISCELLANEOUS:IncontrasttootherH2Ahistones,itdoesnotcontaintheconservedresiduesthatarethetargetofpost-translationalmodifications. SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily. |
| MolecularWeight | ~17kDaobserved |
| PhysicochemicalInformation |
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| Dimensions |
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| MaterialsInformation |
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| MaterialsInformation |
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