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Novus/Recombinant Human TNF-alpha Protein/500ug/NBP2-35076-500ug

Description
Asingle,non-glycosylatedBIOLOGicallyactivepolypeptidechaincorrespondingto158residuesofTNF.

Source:E.coli

AminoAcidSequence:MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

PreparationMethod
Novus"biologicallyactiveproteinsarestringentlypurifiedtoprovideonlythesafestandmosthighlyeffectiveproteinsavailable.ThisproteinwasexpressedinE.coli,purifiedbyHPLC,QCtestedbySDS-PAGEandWesternBlotandvalidatedonappropriatecelllinesforbioactivity.AllHPLCandbioactivitydataisprovidedforyourassurance.
DetailsofFunctionality
TNFalphaproteinisfullybiologicallyactivewhencomparedtostandard.TheED50asdeterminedbyacytotoxicityassayusingmurineL929cellsislessthan0.05ng/ml,correspondingtoaspecificactivityof>2.0x10^7IU/mginthepresenceofactinomycinD.
Source
E.coli
Protein/PeptideType
RecombinantProtein
Gene
TNF
Purity
>98%purebySDS-PAGEandHPLC
EndotoxinNote
Lessthan1EU/ugofendotoxinasdeterminedbyLALmethod.
TheoreticalMW
17.5kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
Publications
ReadPublicationsusingNBP2-35076inthefollowingapplications:
  • Bioactivity
    1publication
  • Flow
    1publication
  • KD
    1publication
  • WB
    1publication
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