产品资料

Novus/FANCD2 Antibody (NB100-182)/NB100-182/0.05 ml

Summary:

Immunogen	HumanFANCD2fusionprotein(N-terminalfragment).[Swiss-Prot#Q9BXW9]
Specificity	ThisantibodyisspecificforFANCD2.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	FANCD2
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions

WesternBlot1:10000-1:20000
SimpleWestern1:25
FlowCytometry
Immunocytochemistry/Immunofluorescence1:200-1:500
Immunohistochemistry2.5-5.0ug/ml
Immunohistochemistry-Paraffin2.5-5.0ug/ml
Immunoprecipitation1:10-1:500
RNAInhibition

ApplicationNotes

ByWesternblot,thisantibodyshouldrecognizeabandat~166kDa(post-translationallymodifiedform).Additionalbandsmaybeseenatlowermolecularweights.ForimmunofluorescenceithasbeentestedinhumanMMCandIRtreatedMEFcells.ThisantibodymayalsobeusedforImmunoprecipitationandImmunohistochemistry-Paraffin.InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue.UseinRNAinhibitionreportedinscientificliterature(PMID:27694619).

PositiveControl

HeLaLysate(NB800-PC1)

ReviewedApplications

Read5Reviewsrated4.4

using
NB100-182inthefollowingapplications:

WesternBlot
Immunofluorescence

Publications

ReadPublicationsusing
NB100-182inthefollowingapplications:

ICC/IF
51publications
IHC
2publications
IHC-P
3publications
IP
5publications
RNAi
1publication
WB
66publications

ReactivityNotes:

NB100-182reactswithhumanandmouseFANCD2.

Packaging,Storage&Formulations:

Storage	Aliquotandstoreat-20Cor-80C.Avoidfreeze-thawcycles.
Buffer	PBS
Preservative	0.05%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

DKFZp762A223
FA4
FACD
FACDFLJ23826
FAD
FAD2
FA-D2
FADFAD2
FANCD
FANCD2
FanconianemiagroupD2protein
Fanconianemia,complementationgroupD2
ProteinFACD2

AlternateNamesfor53BP1Antibody:

Fanconianemia(FANC)isahumanautosomal-recessivecancersusceptibilitydisordercharacterizedbycongenitaldefects,progressivebonemarrowfailure,andcellularhypersensitivitytomitomycinC(MMC).TheFANCsubunitD2proteinisvitalforcellularresistancetoDNAcross-linkingandthearrestofDNAsynthesisafterionizingrADIation(IR).DNAdamageactivatesthemonoubiquitinationofFANCD2,targetingnuclearfocicontainingtheBRCA1protein.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

Fanconianemia(FANC)isahumanautosomal-recessivecancersusceptibilitydisordercharacterizedbycongenitaldefects,progressivebonemarrowfailure,andcellularhypersensitivitytomitomycinC(MMC).TheFANCsubunitD2proteinisvitalforcellularresistancetoDNAcross-linkingandthearrestofDNAsynthesisafterionizingradiation(IR).DNAdamageactivatesthemonoubiquitinationofFANCD2,targetingnuclearfocicontainingtheBRCA1protein.

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