Novus/Recombinant Human FGF basic/FGF2 Protein (NBP2-34921)/NBP2-34921-50ug/50 ug

Summary:

Description	ABIOLOGicallyactiveproteintoFGF2. Source:E.coli AminoAcidSequence:MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Preparation Method	Novus"biologicallyactiveproteinsarestringentlypurifiedtoprovideonlythesafestandmosthighlyeffectiveproteinsavailable.ThisproteinwasexpressedinE.coli,purifiedbyHPLC,QCtestedbySDS-PAGEandWesternBlotandvalidatedonappropriatecelllinesforbioactivity.AllHPLCandbioactivitydataisprovidedforyourassurance.
DetailsofFunctionality	FGFbasic/FGF2proteinisfullybiologicallyactivewhencomparedtostandard.TheED50asdeterminedbyacellproliferationassayusingmurinebalb/c3T3cellsislessthan0.05ng/ml,correspondingtoaspecificactivityof>2.0x10^7IU/mg
Source	E.coli
Protein/PeptideType	BiologicallyActiveProtein
Gene	FGF2
Purity	>95%purebySDS-PAGE
EndotoxinNote	Lessthan1EU/ugofendotoxinasdeterminedbyLALmethod.

Applications/Dilutions:

TheoreticalMW	16.5kDa. Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
ReviewedApplications	Read2Reviewsrated5 using NBP2-34921inthefollowingapplication:
Publications	ReadPublicationusingNBP2-34921.

Packaging,Storage&Formulations:

Notes:

• basicfibroblastgrowthfactorbFGF
• Basicfibroblastgrowthfactor
• bFGF
• FGFbasic
• FGF2
• FGF-2
• FGF2AS
• FGFBprostatropin
• fibroblastgrowthfactor2(basic)
• GFG1
• HBGF-2
• HBGH-2
• heparin-bindinggrowthfactor2
• NUDT6
• Prostatropin

AlternateNamesfor53BP1Antibody:

HumanFGF-2(bFGF),encodedbytheFGF2gene,isamemberofthefibroblastgrowthfactor(FGF)family.Fibroblastgrowthfactorwasfoundinpituitaryextractsin1973andthentestedinabioassaythatcausedfibroblaststoproliferate.AfterfurtherfractionatingtheextractusingacidicandbasicpH,twodifferentformshaveisolatedthatnamed"acidicfibroblastgrowthfactor"(FGF-1)and"basicfibroblastgrowthfactor"(FGF-2).HumanFGF-2(bFGF)shares54%aminoacidsequenceidentitywithaFGF.AffinitybetweenFGF-2(bFGF)anditsreceptorscanbeincreasedbyheparinorheparansulfateproteoglycan.FGF-2(bFGF)playsanimportantroleintheregulationofcellsurvival,celldivision,angiogenesis,celldifferentiationandcellmigration.FGF-2(bFGF)arealsoinvolvedinavarietyofbiologicalprocesses,includingembryonicdevelopment,morphogenesis,tissuerepair,tumorgrowthandinvasion.Additionally,FGF-2(bFGF)isfrequentlyusedforacriticalcomponentofcellculturemedium,e.g.,humanembryonicstemcellculturemedium,serum-freeculturesystems.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.Peptidesandproteinsareguaranteedfor2yearsfromdateofreceipt.

Limitations:

HumanFGF-2(bFGF),encodedbytheFGF2gene,isamemberofthefibroblastgrowthfactor(FGF)family.Fibroblastgrowthfactorwasfoundinpituitaryextractsin1973andthentestedinabioassaythatcausedfibroblaststoproliferate.AfterfurtherfractionatingtheextractusingacidicandbasicpH,twodifferentformshaveisolatedthatnamed"acidicfibroblastgrowthfactor"(FGF-1)and"basicfibroblastgrowthfactor"(FGF-2).HumanFGF-2(bFGF)shares54%aminoacidsequenceidentitywithaFGF.AffinitybetweenFGF-2(bFGF)anditsreceptorscanbeincreasedbyheparinorheparansulfateproteoglycan.FGF-2(bFGF)playsanimportantroleintheregulationofcellsurvival,celldivision,angiogenesis,celldifferentiationandcellmigration.FGF-2(bFGF)arealsoinvolvedinavarietyofbiologicalprocesses,includingembryonicdevelopment,morphogenesis,tissuerepair,tumorgrowthandinvasion.Additionally,FGF-2(bFGF)isfrequentlyusedforacriticalcomponentofcellculturemedium,e.g.,humanembryonicstemcellculturemedium,serum-freeculturesystems.