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| Description | ABIOLOGicallyactivedisulfide-linkedhomodimericproteinconsistingoftwo166residuespolypeptidechainswithMetatN-terminalofVEGFA.
Source:E.coli AminoAcidSequence:MAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR Preparation | Method Novus"biologicallyactiveproteinsarestringentlypurifiedtoprovideonlythesafestandmosthighlyeffectiveproteinsavailable.ThisproteinwasexpressedinE.coli,purifiedbyHPLC,QCtestedbySDS-PAGEandWesternBlotandvalidatedonappropriatecelllinesforbioactivity.AllHPLCandbioactivitydataisprovidedforyourassurance. DetailsofFunctionality | VEGF165proteinisfullybiologicallyactivewhencomparedtostandard.TheED50asdeterminedbyacellproliferationassayusinghumanumbilicalveinendothelialcells(HUVEC)isbetween1.0-8.0ng/ml. Protein/PeptideType | BiologicallyActiveProtein Gene | VEGFA Purity | >95%purebySDS-PAGE EndotoxinNote | Lessthan1EU/ugofendotoxinasdeterminedbyLALmethod. Applications/Dilutions:
Packaging,Storage&Formulations:Thislyophilizedpreparationisstableat2-8degreesC,butshouldbekeptat-20degreesCforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationismoststableat-20to-80degreesC,andcanbestoredforoneweekat2-8degreesC.FormaximalstABIlity,apportionthereconstitutedpreparationintoworkingaliquotsandstoreat-20degreesCto-80degreesC.Avoidrepeatedfreeze/thawcycles. Notes:
AlternateNamesfor53BP1Antibody:VascularEndothelialGrowthFactorisasub-familyofgrowthfactorsproducedbycells,whichstimulatesvasculogenesisandangiogenesis.VEGF"snormalfunctionistocreatenewbloodvesselsduringembryonicdevelopment,newbloodvesselsafterinjury,musclefollowingexercise,andnewvessels(collateralcirculation)tobypassblockedvessels.Humansexpressalternatelysplicedisoformsof121,145,165,183,189,and206aminoacids(a.a.)inlength.VEGFproductioncanbeinducedincellsthatarenotreceivingenoughoxygen.VEGF165appearstobethemostabundantandpotentisoform,followedbyVEGF121andVEGF189.RecombinanthumanVEGF165contains166aminoacidsresiduesanditisadisulfide-linkedhomodimer.Inaddition,itshares88%a.a.withcorrespondingregionsofmouseandrat,96%withporcine,95%withcanine,and93%withfeline,equineandbovineVEGF,respectively.Background:Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.Peptidesandproteinsareguaranteedfor2yearsfromdateofreceipt.Limitations:VascularEndothelialGrowthFactorisasub-familyofgrowthfactorsproducedbycells,whichstimulatesvasculogenesisandangiogenesis.VEGF"snormalfunctionistocreatenewbloodvesselsduringembryonicdevelopment,newbloodvesselsafterinjury,musclefollowingexercise,andnewvessels(collateralcirculation)tobypassblockedvessels.Humansexpressalternatelysplicedisoformsof121,145,165,183,189,and206aminoacids(a.a.)inlength.VEGFproductioncanbeinducedincellsthatarenotreceivingenoughoxygen.VEGF165appearstobethemostabundantandpotentisoform,followedbyVEGF121andVEGF189.RecombinanthumanVEGF165contains166aminoacidsresiduesanditisadisulfide-linkedhomodimer.Inaddition,itshares88%a.a.withcorrespondingregionsofmouseandrat,96%withporcine,95%withcanine,and93%withfeline,equineandbovineVEGF,respectively.
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