产品资料

Novus/LC3B Antibody (1251A) (NBP2-46892)/NBP2-46892SS/0.025 mg

Summary:

AdditionalInformation	RecombinantMonoclonalAntibody
Immunogen	AsyntheticpeptidemadetoanN-terminalportionofthehumanLC3proteinsequence(betweenresidues1-100).[UniProt#Q9GZQ8].
Localization	LC3-Iiscytoplasmic.LC3-IIbindstotheautophagicmembranes.
Marker	Autophagosomes
Specificity	SpecificforLC3B.
PredictedSpecies	Rat(94%),Porcine(100%),Bat(100%),Xenopus(94%),Zebrafish(100%),Bovine(100%),Fish(100%).Backedbyour100%Guarantee.
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Gene	MAP1LC3B
Purity	ProteinGpurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions

WesternBlot1.0-2.0ug/ml
Immunocytochemistry/Immunofluorescence10-20ug/ml
Immunohistochemistry1:100-1:500
Immunohistochemistry-Paraffin1:100-1:500
Immunoprecipitation2-10ug
Flow(Intracellular)1-2.5ug/mL
KnockoutValidated

ApplicationNotes

InWesternblotthisLC3BrecombinantmonoclonalantibodydetectsbothLC3IandLC3IIwithChloroquinetreatment.WithICCautophagosomestainingwasobservedaftertreatmentwithChloroquine.

PositiveControl

LC3BLysate(NBL1-12844)
Neuro2aLysate(NBP2-49688)
HeLaLysate(NBP2-49689)

ReviewedApplications

Read3Reviewsrated5

using
NBP2-46892inthefollowingapplications:

WesternBlot

Publications

ReadPublicationsusing
NBP2-46892inthefollowingapplications:

FLOW
1publication
WB
1publication

Packaging,Storage&Formulations:

Apg8b
ATG8F
Autophagy-relatedproteinLC3B
Autophagy-relatedubiquitin-likemodifierLC3B
lc3bautophagymarker
LC3Bmonoclonal
LC3B
lc3-i,ii
LC3II
MAP1lightchain3-likeprotein2
MAP1A/1BLC3
MAP1A/MAP1BLC3B
MAP1LC3B
microtubuleassociatedprotein3b
microtubuleassociatedprotein3beta
microtubule-associatedprotein1lightchain3betaMAP1A/MAP1Blightchain3B
microtubule-associatedproteins1A/1Blightchain3
microtubule-associatedproteins1A/1Blightchain3B

Notes:

LC3(microtubule-associatedproteinlightchain3),themoststudiedautophagybiomarker,wasoriginallyidentifiedasasubunitofmicrotubule-associatedproteins1Aand1B(MAP1LC3)andwaslaterfoundtocontainsimilaritytoyeastproteinApg8/Aut7/Cvt5.Distributedubiquitouslyineukaryotes,LC3isexpressedas3splicevariants/isoforms(LC3A,LC3BandLC3C)whichundergopost-translationalprocessing,wherein,theunprocessedformofLC3isproteolyticallycleavedbyAtg4proteasetoformLC3-Iwithcarboxyterminalexposedglycine.Duringautophagy,thisexposedglycineofLC3-IisconjugatedbyAtg7(anE1-likeactivity),Atg3(anE2-likeconjugatingactivity)andbyAtg12-Atg5-Atg16Lmultimers(E3-likeligaseactivity)tophosphatidylethanolamine(PE)moietyforgeneratingLC3-II.ThelipophiliccharacterofPEgroupfacilitatesLC3-IIinsertionintoautophagosomesmembranes,andasaresultLC3-IIisdegradedwhenautophagosomesfusewithlysosomestoformautolysosomesforlysusofintra-autophagosomalcomponentsbylysosomalhydrolases.ConversionofLC3ItoLC3IIwhencorrelatedwithautophagosomenumbersisconsideredasthebestmarkerofautophagybecauseLC3-IIistheonlywell-characterizedproteinwhichspecificallylocalizetoautophagicstructuresthroughoutautophagy(fromphagophoretolysosomaldegradation).LC3isagreattoolinresearchasautophagyisimplicatedinnumerousphysiological/pathologicalprocessesincludingresponsestoexercise/aging,cancer,metabolicandneurodegenerativedisorders,andcardiovascular/pulmonarydiseases.

AlternateNamesfor53BP1Antibody:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations: