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Agrenvec/Anti-Human Activin B (IgG) – RF0054/RF0054/100-microg

Description:

 RabbitPolyclonalAntibody(IgG)toActivinB

Application:

 WB,ELISA

Reactivity:

 Human

Conjugate:

 Unconjugated

Description: Anti-HumanActivinBIgGisdevelopedinrabbitandpurifiedbyaffinitychromatographyonproteinG(>98%purity).

Immunogen: Highlypure(>97%)recombinanthumanActivinBexpressedinplants.

Applications:

    • ELISA: Suggestedstartingdilution1/500.Thisantibody,inconjunctionwithcompatIBLesecondaryreagent(anti-rabbitIgG-APconjugate),allowsthedetectionof <10ng/wellofrhumanActivinB(RF0029).
    • WesternBlot: Suggestedstartingdilution1/500.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.
rf0054
Fig1.WesternBlotanalysisofrhumanActivinBusingAnti-HumanActivinBIgG(RF0054)
HumanActivinBproteinwasresolvedbySDS-PAGE,transferredtoaNCmembraneandprobedwithadilution1:500ofAnti-HumanActivinBIgG.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)wasusedassecondaryreagent.MWM:molecularweightMarker;Lane1:0.1ugofrHActivinB(monomeric form);Lane2:0.3ugofrHActivinB(multimericform).
Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisnotnecessarilyexcludesitsuseinsuchprocedures.Optimaldilutionconditionsshouldbedeterminedbythefinaluser.Bulksizesareavailableuponrequest.ForR&Dpurposesonly.PurchasermustdeterminethesuitABIlityoftheproduct(s)fortheirparticularuse
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