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Themajorityofcalciumrequiredformusclecontractionismobilisedfromthesarcoplasmicreticulum(SR),towhichithastoreturntoinordertofacilitaterelaxation.The(Ca2+-Mg2+)ATPaseenzymeisresponsIBLeforthesequestrationofcalciumafteritsreleasefromtheSR,andiscontrolledbyphospholamban(PLB)incardiac,smoothandslow-twitchskeletalmuscle.
Themonoclonalantibody(A1)wasraisedagainstbovinecardiacPLB andrecognisesanepitopecomprisingofresidues7LTRSAIRRAS16. TheantibodyreactswithallformsofphospholambanPLB:monomer,oligomers,phosphorylatedandnon-phosphorylated.
TheantibodystimulatesCa2+pumpactivityuponbindingtoPLB,toanextentequivalenttostoichiometricphosphorylationofPLBManipulationofSERCA2:PLBratiosisatherapeuticstrategyforheartfailure.EliminationofPLBboostsSRCa2+-loadwhichcanexacerbatepathologicaloutcomes(apoptosis,arrhythmias)whencombinedwithotheralterationsinSRfunction.
Testedapplications:Westernblot(1:5000dilution),IHCMicroscopy(1:200dilution),Ca2+pumpassays(1:500dilution).
| Description | Lyophilisedrabbitpolyclonalanti-serumcontainingIgGantibodyspecificforser-16phosphorylatedformsofPLB. |
| Immunogen | Phosphopeptidecomprisingresidues9-19-Y(residuesR9SAIRRAS(PO3H2)TIE19Y)conjugatedtoKLH. |
| Isotype | IgG |
| Purification | Rawserum |
| Speciesreactivity | ReactswithphosphoSer-16ofPLBfromhuman,mouse,rat,rabbit,chicken,ferret,hamsterandsheep. |
| Testedapplications | WB,ELISAandIHCmicroscopy |
| Recommendeddilution | WB1:5000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin25µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
| Storage | Lyophilised:4°CReconstituted:-80°C |
| Datasheet | A010-14 |
| MSDS | A010-14 |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |
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