43kDaDystrophin-associatedglycoprotein;(43-DAG,DG,b-DAG,b-DG,DAG1)β-dystroglycanisacomponentofthedystrophinglycoproteincomplex(DGC),amembrane-spanningcomplexthatlinkstheinteriorCytoskeletontotheextracellularmatrixinmuscleandothertissues(Winder2001).Itisatransmembraneprotein(singleTMdomain)of34.9kDamolecularweight,andpI8.85,whichdisplaysanapparentmolecularweightof43kDabySDS-PAGEduetoasparagine-linkedglycosylationandahighproportionofprolineresiduesinthecytoplasmicdomain.ThedystroglycancomplexisasubcomplexwithintheDGCcomprisingalphaandbeta-subunits.Dystroglycanisofimportanceincellsignalling,cellpolarity,celladhesionandmovement.β-dystroglycanbindstodystrophin(inmusclecells)andutrophin(innon-musclecells),butdissociatesfromtheseproteinswhenphosphorylatedonTyr-892allowingitsassociationwithSH2containingproteinssuchasSrcandothercytOlinkerproteinssuchasplectin.ThisantibodyrecognisesTyr-892phosphorylatedformsofβ-dystroglycan(Tyr-892inhuman,Tyr-890inmouse)
Testedapplications:Westernblot(1:500dilution),IHCMicroscopy(1:50dilution),Immunoprecipitation(1:10dilution)inRIPAbuffer.Speciesrecognition:recognisesβ-dystroglycaninallmammalianspecies,whenphosphorylatedonTyr-892(orequivalent).
Description | Lyophilisedrabbitpolyclonalanti-serumcontainingantibodyspecificforTyr-892phosphorylatedformsofβ-dystroglycan |
Immunogen | Phosphopeptidecomprisingresidues983-994(residuesMTPYRSPPPY(PO3H2)VP)conjugatedtoKLH. |
Isotype | IgG |
Purification | Rawserum |
Speciesreactivity | Recognisesβ-dystroglycaninallmammalianspecies,whenphosphorylatedonTyr-892(orequivalent). |
Testedapplications | WB,IH,IP |
Recommendeddilution | WB:1:500,IH1:50,IP1:10(inRIPAbuffer) |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-200 |
MSDS | A010-200 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |
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