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Badrilla/Ryanodine receptor 2 (RYR2) (pSer2814) pAb serum/A010-31/50 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Badrilla/Ryanodine receptor 2 (RYR2) (pSer2814) pAb serum/A010-31/50 µl

DESCRIPTION:

Afullyvalidated,highlyspecificrabbitpolyclonalantibodytoSerine-2814phosphorylatedRyanodineReceptor2

TheRyanodineReceptor(RyR2)isaCa2+channelofcardiacmusclethatplaysacentralroleinECcoupling.ThebindingofCa2+toRyanodineReceptor2RyR2opensthechannelandCa2+storedintheSRmovesthroughthechannelintothecytosoltoinitiatemusclecontraction.CaMKII,wasabletophosphorylateSer-2814ofRYR2enhancingCa2+-sensitivityandincreasingopenprobABIlity.ExcessivephosphorylationofSer-2814leadstoatrialfibrillation,arrhythmiasandsuddendeath

Testedapplications: Westernblot(1:5000dilution),immunofluorescencemicroscopy(1:100). Speciesrecognition: Allmammalianspecies,whenphosphorylatedonSer-2814(orSer-2815rabbit)Antibodyrecognisesepitopedespitesingleresiduedifferenceinepitopebetweenspecies(V2815I;humanvsmouse).

 

DATASHEET:

DescriptionLyophilisedrabbitanti-serum
ImmunogenSyntheticpeptide(TSQVS(PO3H2)VDAAH2819)correspondingtoaminoacidssurroundingthephosphorylatedserineresidueatposition2814ofRyR2(human)conjugatedtoKLH.
IsotypeIgG
PurificationRawserum
SpeciesreactivityPeptidealignswithRyR2fromallmammalianspecies.Sequencecorrespondsexactlyinrabbitbutdiffersatoneresidue(V2815I)inmouseandrat.Howeverantibodyrecognisesbothsequences.
TestedapplicationsWB,IHC
RecommendeddilutionWB1:5000,IHC1:100
OptimizationOptimaldilutionstobedeterminedbyenduser
StorageLyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles.
StorageLyophilised:4°CReconstituted:-80°C
DatasheetA010-31Lot1209-04

A010-31Lot0513-06
MSDSA010-31
RegulatorystatementProductforresearchuseonly.Notintendedfordiagnosticortherapeuticuse.
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