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Background: Phospholamban(PLB/PLN)isasmalltransmembraneproteinwhichplaysanimportantroleincontrollingtheactivityofthesarcoplasmicreticulumATPase(SERCA2a)ofcardiacmuscleduringcalciumsequestration.PhospholambanisphosphorylatedonseparateaminoacidresiduesbycAMP-dependent,andcGMP-dependent(Ser-16) andCa2+/CaM-dependent(Thr-17) proteinkinasesinresponsetoβ-adrenergicstimulation. AkthasalsobeenshowntophosphorylateThr-17.Theresultisanincreasedcalciumpumpactivitywhichreducesthetimecourseofthecalciumtransient,increasesthecalciumloadinthesarcoplasmicreticulum,andconsequently,producesalargercalciumtransientatthenextactionpotential. However,alterationinthishomeostaticinteractionhasbeenshowntoresultinheartfailure
Testedapplications: WesternBlot(1:5000dilution),ELISA. Speciesrecognition:allmammalianspecies,whenphosphorylatedonSer-16.
| Description | LyophilisedRabbitpolyclonalanti-serum(A010-12)containingIgGantibodyspecificforSer-16phosphorylatedformsofPLB(Drago&Colyer,1994). |
| Immunogen | Phosphopeptidecomprisingresidues9-19-Y(residuesR9SAIRRAS(PO3H2)TIE19Y)conjugatedtoKLH. |
| Isotype | IgG |
| Purification | Rawserum |
| Speciesreactivity | Theantibodyrecognisesmonoandoligomericphospholambanwhenphosphorylatedonserine-16byPKA.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthePLBPhosphoSer-16epitope.AntibodyaffinityisreducedincircumstancesofdualphosphorylationofSer-16andThr-17. |
| Testedapplications | WB,ELISAandIHCmicroscopy |
| Recommendeddilution | WB1:5000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin25µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
| Storage | Lyophilised:4°CReconstituted:-80°C |
| Datasheet | A010-12 |
| MSDS | A010-12 |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |
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