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Badrilla/Phospholamban (PLN, PLB) (pSer16) pAb serum/A010-12/25 μl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Badrilla/Phospholamban (PLN, PLB) (pSer16) pAb serum/A010-12/25 μl

DESCRIPTION:

Afullyvalidated,highlyspecificpolyclonalantiserumtoSerine-16phosphorylatedformsofphospholamban

Background: Phospholamban(PLB/PLN)isasmalltransmembraneproteinwhichplaysanimportantroleincontrollingtheactivityofthesarcoplasmicreticulumATPase(SERCA2a)ofcardiacmuscleduringcalciumsequestration.PhospholambanisphosphorylatedonseparateaminoacidresiduesbycAMP-dependent,andcGMP-dependent(Ser-16) andCa2+/CaM-dependent(Thr-17) proteinkinasesinresponsetoβ-adrenergicstimulation. AkthasalsobeenshowntophosphorylateThr-17.Theresultisanincreasedcalciumpumpactivitywhichreducesthetimecourseofthecalciumtransient,increasesthecalciumloadinthesarcoplasmicreticulum,andconsequently,producesalargercalciumtransientatthenextactionpotential. However,alterationinthishomeostaticinteractionhasbeenshowntoresultinheartfailure

Testedapplications: WesternBlot(1:5000dilution),ELISA. Speciesrecognition:allmammalianspecies,whenphosphorylatedonSer-16.

 

DATASHEET:

DescriptionLyophilisedRabbitpolyclonalanti-serum(A010-12)containingIgGantibodyspecificforSer-16phosphorylatedformsofPLB(Drago&Colyer,1994).
ImmunogenPhosphopeptidecomprisingresidues9-19-Y(residuesR9SAIRRAS(PO3H2)TIE19Y)conjugatedtoKLH.
IsotypeIgG
PurificationRawserum
SpeciesreactivityTheantibodyrecognisesmonoandoligomericphospholambanwhenphosphorylatedonserine-16byPKA.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthePLBPhosphoSer-16epitope.AntibodyaffinityisreducedincircumstancesofdualphosphorylationofSer-16andThr-17.
TestedapplicationsWB,ELISAandIHCmicroscopy
RecommendeddilutionWB1:5000
OptimizationOptimaldilutionstobedeterminedbyenduser
StorageLyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin25µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles.
StorageLyophilised:4°CReconstituted:-80°C
DatasheetA010-12
MSDSA010-12
RegulatorystatementProductforresearchuseonly.Notintendedfordiagnosticortherapeuticuse.
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