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Badrilla/SERCA1/2 mAb (clone Y/1F4)/A010-21AP/50 μl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Badrilla/SERCA1/2 mAb (clone Y/1F4)/A010-21AP/50 μl

DESCRIPTION:

Afullyvalidated,highlyspecificaffinitypurifiedmousemonoclonalantibodytoSERCAisoforms1and2(anti-SERCA1/2)

SERCA1istheCa2+-pump(109kDa)locatedinthesarcoplasmicreticulum(SR)offast-twitchskeletalmuscleandSERCA2istheCa2+-pumplocatedinthesarcoplasmicreticulum(SR)ofcardiac,smoothandslow-twitchskeletalmuscle.Theenzymetransports2Ca2+fromthecytoplasmintotheSRusingthehydrolysisof1moleculeofATPtopowerthereaction.Thisterminatesmusclecontractionandpromotesmusclerelaxation.MutationsinSERCA1areassociatedwithBrodydisease;mutationsinSERCA2areassociatedwithDarierdiseaseanddownregulationofSERCA2isseeninheartfailure.ThemonoclonalantibodyY/1F4wasproducedbyimmunizingmicewithpurifiedSERCA1:theantibodyinhibitsSERCA1activityuponbinding(Colyeretal.,1989).AntibodyY/1F4recognisesbothSERCA1andSERCA2.

TestedApplications:Westernblot(1:2000dilution),IHCMicroscopy(1:50dilution).Speciesrecognition:Testedonrattissue,butimmunogenicpeptidealignswithMammalian,Avian,AmphibianandFishSERCA

DATASHEET:

DescriptionAffinitypurifiedmousemonoclonalantibodytoSERCAisoforms1and2
ImmunogenSyntheticpeptide(VGNKMFVKGA)correspondingtoaminoacids508-517ofSERCA1,conjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage.
IsotypeIgG
PurificationProteinAaffinitypurified
SpeciesreactivityTestedonRattissue,butimmunogenicpeptidealignswithMammal,Avian,AmphibianandFishSERCA
TestedapplicationsWB
RecommendeddilutionWB1:2000
OptimizationOptimaldilutionstobedeterminedbyenduser
StorageLyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles.
StorageLyophilised:4°C.Reconstituted:-80°C
DatasheetA010-21AP
MSDSA010-21AP
RegulatorystatementProductforresearchuseonly.Notintendedfordiagnosticortherapeuticuse.
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