CnT-Primeisafullydefined,animal-component-freeculturemediumforisolationandexpansionofepithelialcellsfromskin,cornea,gingiva,mammaryandbladdertissue.Itwasdevelopedusinghumantissue,butmayalsobeusedwithotherspecies(e.g.mouse).
Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.
ItisanevolutionandrefinementofourpreviousPCTmediasuchasCnT-07,CnT-20,andCnT-24.
Itusesanoptimsedbasalmediumwithadditionaltraceelements,protectiveantioxidantsandvitamins.
ThebasalmediumisalsosupplementedwitharangeofProgenitorCellTareted(PCT)growthfactorstoextendlongevity,andco-factorstoimprovegrowthfactorbindingtomembrane-boundreceptors.
Formaximumisolationefficiencyfromepithelialtissues,itisrecommendedtosupplementCnT-PRwiththeIsoBoostsupplement(#CnT-ISO-50),whichhasbeenfoundtodoublecellyieldinthefirstweekofculture.
PrimaryepithelialcellsgrowinginCnT-PRretaindeliverextendedgrowth.Fordifferentiationexperiments,itisrecommendedtoswitchtotheCnT-PR-Ddifferentiationmedium.
ThemediumundergoarangeofQCtestsinourlabbeforebeingreleasedforsale.Pleaseseethedatasheetfordetails.
Liketotryatestsample?Clickhereformoreinformation.
Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowth.PleasevisitourProtocolsPageforourrecommendations.
PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCELLnTECprimarycellsandmediainthisspecificfield.
CnT-Primeisafullydefined,lowcalcium(0.07mM)mediumformulationdesignedforoptimalgrowthandlongevityofprimaryepithelialcells(skin,cornea,oral,mammary).Itiscompletelyfreeofanimalorhuman-derivedcomponents.CnT-Primecontainsafullrangeofaminoacids,minerals,vitaminsandorganiccompounds,plusgrowthfactorstodeliverstrongcellproliferation,andcofactorstoimprovegrowthfactorbindingtocellsurfacereceptors.CnT-PrimealsocontainsPCTfactorsthatmaximiseretentionofproliferativeprogenitorcells,increaselongevity,andminimiselossthroughdifferentiation.Itdoesnotcontainphenol-red,orantibiotics/antimycotics.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn