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CellnTec/CnT-PR/CnT-PR/1 Ea

CnT-Primeisafullydefined,animal-component-freeculturemediumforisolationandexpansionofepithelialcellsfromskin,cornea,gingiva,mammaryandbladdertissue.Itwasdevelopedusinghumantissue,butmayalsobeusedwithotherspecies(e.g.mouse).

Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.

ItisanevolutionandrefinementofourpreviousPCTmediasuchasCnT-07,CnT-20,andCnT-24.

Itusesanoptimsedbasalmediumwithadditionaltraceelements,protectiveantioxidantsandvitamins.

ThebasalmediumisalsosupplementedwitharangeofProgenitorCellTareted(PCT)growthfactorstoextendlongevity,andco-factorstoimprovegrowthfactorbindingtomembrane-boundreceptors.

Formaximumisolationefficiencyfromepithelialtissues,itisrecommendedtosupplementCnT-PRwiththeIsoBoostsupplement(#CnT-ISO-50),whichhasbeenfoundtodoublecellyieldinthefirstweekofculture.

PrimaryepithelialcellsgrowinginCnT-PRretaindeliverextendedgrowth.Fordifferentiationexperiments,itisrecommendedtoswitchtotheCnT-PR-Ddifferentiationmedium.

ThemediumundergoarangeofQCtestsinourlabbeforebeingreleasedforsale.Pleaseseethedatasheetfordetails.

Liketotryatestsample?Clickhereformoreinformation.

Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowth.PleasevisitourProtocolsPageforourrecommendations.

PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCELLnTECprimarycellsandmediainthisspecificfield.


CnT-Prime,EpithelialCultureMedium

CnT-Primeisafullydefined,lowcalcium(0.07mM)mediumformulationdesignedforoptimalgrowthandlongevityofprimaryepithelialcells(skin,cornea,oral,mammary).Itiscompletelyfreeofanimalorhuman-derivedcomponents.CnT-Primecontainsafullrangeofaminoacids,minerals,vitaminsandorganiccompounds,plusgrowthfactorstodeliverstrongcellproliferation,andcofactorstoimprovegrowthfactorbindingtocellsurfacereceptors.CnT-PrimealsocontainsPCTfactorsthatmaximiseretentionofproliferativeprogenitorcells,increaselongevity,andminimiselossthroughdifferentiation.Itdoesnotcontainphenol-red,orantibiotics/antimycotics.

Species
Developedforprimaryhumanepithelialcells.Maybeusedforotherspeciesaswell,includingmouse.
Tissuetype
Epithelia
Packsize
500mLbottle,fullysupplementedwithgrowthfactors.Nofurtheradditionsrequired.
Productuse
Developedspecificallytodeliverstrongproliferationandextendedlongevityofprimaryepithelialcellsinafullydefinedenvironment.TheinclusionofPCTfactorsincreasesretentionofproliferativeprogenitorcells,andminimiselossthroughdifferentiation.Notrecommendedforusewheninducingdifferentiation.Fordifferentiationwork,pleaseuseCnT-PR-Dmedium.WhenusingCnT-PRforisolation,additionoftheIsoBoostsupplement(#CnT-ISO-50)hasbeenfoundtodoublecellyields.
MediaType
2D-Prolif
Defined
Yes
ACF
Yes
Culturecondition
Thismediumisdesignedforuseina5%CO2atmosphere.Forsuggestedisolation,passaging,differentiationandfreezingprotocols,pleasevisittheresourcessectionofwww.cellntec.com
Note
Forroutinecellcultivation,CELLnTECdoesnotrecommendtheuseofantibiotics/antimycotics.Duringisolation,antibiotics/antimycoticsarerecommendedupuntiltheendofP1.
Storage/Shelflife
Storefrozenbelow-15oC.Forbestbeforedate,seelabel.Topreparemediumforuse,itispreferabletothawovernightat4oC.Alternatively,mediummaybethawedatroomtemperatureforapprox5-6hours.Ifthawingatroomtemperature,ensurecontentsdonotriseabove15oCattheendofthethawingprocess.Donotthawinahotwaterbath.Oncethawed,mediumhasaremainingshelf-lifeof6-weekswhenstoredat4oCinthedark.Certainculturemediacomponentsareverysensitivetolight.Minimizelightexposureatalltimes.
Qualitycontrol
Mediacompositionistestedviaosmolality,pHandtheconcentrationofvariousions.Mediafunctionalityistestedbyevaluatinggrowthandmorphologyorprimaryhumanepithelialcellsoveratleast2passages.Freeofbacteria,fungiandmycoplasmacontamination.
Shippingcondition
Mediumisshippedfrozen.
Intendeduse
Forresearchuseonly.Notforuseintherapyordiagnostics.
Lastupdate
2017-09-27
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