CellnTec/CnT-PR-3D/CnT-PR-3D/1 Ea

CnT-Prime3DBarrierisafullydefined,animal-component-freemediumdesignedtodelivermaximaldifferentiationandincreasedbarrierfunctionin3Depidermalkeratinocytemodels.Itwasdevelopedusinghumantissue,butmayalsobeusedwithotherspecies(e.g.mouse).

Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.

Itisanevolutionandrefinementofourprevious3Ddifferentiationmedium,CnT-02-3DP.

Itusesanoptimsedbasalmediumwithadditionaltraceelements,protectiveantioxidantsandvitamins.

Thismediumisspecificallydesignedtocreateanenvironmentconducivetofulldifferentiation,andtheformationofastrongepidermalbarrier(moreinfo).ItdoesnotcontainPCTgrowthfactors,orotherproliferativefactorsthatretarddifferentiation.

CnT-PR-3Dmediumisusedtoestablish3DepidermalmodelsusingprimaryhumankeratinocytesgrowinginCnT-Prime(CnT-PR)medium.Thiscanbeachievedwith12daysofair-liftcultureaccordingtoourrecommendedprotocol(seeprotocollinkbelow).

The3DBarriermediumisalsoavailableaspartofour3DStarterkits,thatcontainallcomponentsnecessaryfortheestablishmentof3Depidermalmodels.

Liketotryatestsample?Clickhereformoreinformation.

ThemediumundergoesarangeofQCtestsinourlabbeforebeingreleasedforsale.Pleaseseethedatasheetfordetails.

Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowthinroutine2Dculture.PleasevisitourProtocolsPageforourrecommendations.

PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCELLnTECprimarycellsandmediainthisspecificfield.

CnT-Prime3DBarrierMedium

CnT-Prime3DBarrierisafullydefinedmediumformulationdesignedforoptimaldifferentiationofprimaryhumanepidermalkeratinocytesin3Dairliftcultures.Itiscompletelyfreeofanimalorhuman-derivedcomponents.CnT-Prime3DBarriercontainsafullrangeofaminoacids,minerals,vitaminsandorganiccompounds,andmodifiedgrowthfactorstodeliverincreasedbarrierfunction.Italsocontainsadditionallipids,whichmayrequirewarmingtoroomtemperaturetore-solubilizeafterthawing(seebelow).Itdoesnotcontainphenol-red,orantibiotics/antimycotics.

Species

Developedforprimaryhumanepidermalkeratinocytes.Maybeusedforotherspeciesaswell,includingmouse.

Tissuetype

Epithelia

Packsize

500mLbottle,fullysupplementedwithgrowthfactors.Nofurtheradditionsrequired.

Productuse

Developedfortheestablishmentoffullystratifiedculturesofprimaryhumanepidermalkeratinocyteswithincreasedbarrierfunction.Foradetailedinstructionsdescribingthedevelopmentofthe3Dmodels,pleaseseethedifferentiationprotocolsintheresourcessectionofwww.cellntec.com.

MediaType

3D-Diff

Defined

Yes

ACF

Yes

Culturecondition

Thismediumisdesignedforuseina5%CO₂atmosphere.Foradetaileddifferentiationprotocol,pleasevisittheresourcessectionofwww.cellntec.com

Note

Forroutinecellcultivation,CELLnTECdoesnotrecommendtheuseofantibiotics/antimycotics.Thisisparticularlyimportantduringdifferentiation.

Storage/Shelflife

Storefrozenbelow-15^oC.Forbestbeforedate,seelabel.Topreparemediumforuse,itispreferabletothawovernightat4^oC.Alternatively,mediummaybethawedatroomtemperatureforapprox5-6hours.Oncethawed,lipidsthathavenotfullyre-solubilisedinthecoldaqueousmediummaybevisIBLeasathinmilkylayeratthebottomofthebottle.Tosolubilizetheselipids,warmmediumtoroomtemperatureandswirlgently.Donotthawmediuminahotwaterbath.Oncethawed,mediumhasaremainingshelf-lifeof6-weekswhenstoredat4^oCinthedark.Certainculturemediacomponentsareverysensitivetolight.Minimizelightexposureatalltimes.

Qualitycontrol

Mediacompositionistestedviaosmolality,pHandtheconcentrationofvariousions.Mediafunctionalityistestedbydifferentiatingprimaryhumanepithelialcellsin3Dairliftculture.Freeofbacteria,fungiandmycoplasmacontamination.

Shippingcondition

Mediumisshippedfrozen.

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Lastupdate

2017-09-27