CellnTec/CnT-DNP-10/CnT-DNP-10/1 Ea

DispaseII(NeutralProtease)isanoptimalemzymeforisolationofprimaryepithelialcells.

Itprovidesgentlebuteffectiveseperationofepithelialandstromalcells,allowingpopulationsofhighpuritytobeseededintoprimaryculture.

Itcleavesfibronectin,collagenIV,andtoalesserextentcollagenI,andcanbeusedtoseparateepithelialtissuesfromunderlyingmesenchyma.

OurdispaseproductCnT-DNP-10isprovidedas5x100mg(approx)powderaliquots.Powderamountsmayvaryfromlot-to-lot,duetodifferingactivities.

Each100mgaliquotissufficienttomakeup40mLof1xsolution,whichissufficientfor2-4isolations(dependingonquantityoftissueused).Final1xconcentrationaftersolubilisationwillbe2.4U/mL.

With5powderaliquotsintotal,CnT-DNP-10isthussufficientforupto20isolations.

Forrecommendedisolationprotocols,pleaseseetheIsolationProtocolspage.

DispaseII,NeutralProtease,5x0.1gram

Dispase®isaneutralproteasewhichcleavesfibronectin,collagenIV,andtoalesserextentcollagenI,andcanbeusedtoseparateepithelialtissuesfromunderlyingmesenchyma.

Tissuetype

Enzymes

Packsize

5x100mg(approx)powderaliquots(separatetubes)

Productuse

Reconstituteasinglepowderaliquotbyadding4mLofPBSdirectlytothetubesupplied,tomakeupa10xsolution.Gentlyswirltoensurecompletesolubilization.Sterilefilterthis10xsolutionwithasyringe-topfilterintoa50mLtube,thenadd36mLofsterilePBStomakeupthe1xsolution.Thequantityofpowderincludedineachaliquotwillvaryslightlyfromlot-to-lot,accordingtothelot-specificactivity.Theamountofpowderincludedwillalwaysdeliverafinal1xactivityof2.4U/mLaftersolubilisation.Thissolutionmaybestoredat4°Cforusewithin2weeks,oraliquotedinto15mLtubesandfrozenat-20°Cforusewithin6months.Forrecommendedisolationprotocols,pleasevisittheresourcessectionofwww.CellnTec.com

Defined

No

ACF

No

Storage/Shelflife

Storeat4°Cinpowderform.Seelabelforexpirydate.AfterreconstitutionwithPBS,CnT-DNP-10shouldbealiquotedintodesiredquantities(e.g.15mL),andthealiquotsrefrozenandstoredat-20°Cforusewithin6months.Avoidadditionalfreezeandthawcycles.Thawaliquotsatat4°C,andstoreatthistemperatureuntiluse.  Usewithin2weeksofthawing.

Qualitycontrol

Testedforsterilityandsuccessfulisolationofprimaryepithelialcells.

Shippingcondition

CnT-DNP-10canbeshippedaloneatambienttemperature,orincludedwithotherfrozenproducts.

Comments

Inhibitors:EDTA;EGTA;Hg2+;heavymetals;notinhibitedbyserumActivators:Ca²⁺;Mg²⁺;Mn²⁺;Fe²⁺;Fe³⁺;Al³⁺pHoptimum:6to8.5

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Activityconcentration

Thefinal1xsolutionhasanactivityofapproximately2.4U/mL

Lastupdate

2015-11-12