CellnTec/CnT-09/CnT-09/1 Ea

CnT-09isa10%serumculturemediumdesignedforefficientisolationandextendedinvitrocultureofcaninekeratinocytes.

Itusesanoptimisedbasalmediumwithadditionaltraceelements,protectiveantioxidantsandvitamins.

ThismediumisalsorecommendedforusewiththeCPEKcaninekeratinocytecellline.

ThemediumundergoarangeofQCtestsinourlabbeforebeingreleasedforsale.Pleaseseethedatasheetfordetails.

Thawing,seedingandpassagingprotocolsareparticularlyimportantforoptimalcellgrowth.PleasevisitourProtocolsPageforourrecommendations.

PleasevisitourpublicationsdatabaseandsearchbytissuetypetoobtainarangeofarticlesthathaveusedCellnTecprimarycellsandmediainthisspecificfield.

EpidermalKeratinocyteMedium

CnT-09isaliquidculturemediumkitincludingbothbasalmediumandsupplements.Itcontainsserum.Itdoesnotcontainantibiotics/antimycotics.Thebasalmediumcontainsaminoacids,minerals,vitaminsandorganiccompounds,butisproteinfree.

Species

Formulatedforcaninekeratinocytes.

Tissuetype

Epithelia

Packsize

CnT-09isa500mLkitconsistingof:

• CnT-BM.2:500mLCnTBasalMedium2(liquid)
• CnT-09.S:containingtwosupplements(SupplementsAandB;deliveredfrozen;tobeaddedto500mLbasalmediumpriortouse) 

Productuse

Developedforimprovedcaninekeratinocytegrowth.

MediaType

2D-Prolif

Defined

No

ACF

No

Culturecondition

Thismediumisdesignedforuseina5%CO₂atmosphere.Forsuggestedcellcultureprotocols,pleaseseeourresourcessection.

Note

Forroutinecellcultivation,CELLnTECrecommendstoworkwithoutantibiotics/antimycotics.

Storage/Shelflife

• CnT-BM.2basalmedium:Storeat4°Cinthedark.Forexpirydate,pleaseseelabelonbottle.Pleasedonotfreeze.
• CnT-09.Ssupplements:Storeat-20°C.Forexpirydate,pleaseseelabelontubeandinbag.
• Thecompletemedium(basalmediumsupplementedwiththecorrespondingsupplements)mustbestoredat4°Cinthedark.Storageinthismannerwillprovideaminimumshelflifeof6weeks.

Important:Thequalityofcellculturemediaisrapidlydegradedbylight.Storeinthedark,andminimizealllightexposureduringhandling.

Qualitycontrol

Mediafunctionalityandcellmorphologytestedbycellcultivationover2passagesinsupplementedmedium.Osmolality,pHandconcentrationofvariousionsaretested.Freeofbacteria,fungiandmycoplasmacontamination.

Shippingcondition

• CnT-BM.2basalmediumisshippedatambienttemperature.
• CnT-09.Ssupplementsareshippedfrozen.

Intendeduse

Forresearchuseonly.Notforuseintherapyordiagnostics.

Lastupdate

2016-02-05