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TheImmunoStaralphamelanocytestimulatinghormoneantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratpituitaryusingbiotin/avidin-HRPtechniques.
Recommendedprimarydilutionis1/4000-1/6000inPBS/0.3%TritonX-100-Bn/Av-HRPTechnique.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith100ug/mLofalpha-MSH.
PhotoDescription:IHCimageofneuronsstainingforMSHintherathypothalamus(above)andratpituitarywithnickelpreparation(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforsectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith:Pig,Hamster,Human,Monkey,Mouse,Rabbit,Rat,Trout
AvailABIlity:InStock
AlternateNames: POMC;proopiomelanocortin,anti-Alpha-MSH
RRID: AB_572218
Immunogen:alpha-MSH
GeneSymbol/ID,Accession#: POMC,5443
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