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Thehistochemicalantibodyfor5-HT1Areceptorisgeneratedinarabbitagainstsyntheticpeptidesequencecorrespondingtoaminoacids294-312oftherat5-HT1Areceptor.Theantiserumisprovidedas100µlofaffinitypurifiedserumcontaining1%BSA.Controls:TheImmunoStar5-HT1Areceptorantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.
Theantiserumdemonstratesstronglypositivelabelingofratcortex,arcuateandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare1/200-1/300inPBS-Bn/Av-HRPdetection.Intensificationmethodssuchasnickelwillapproximatelydoublethedilutionfactorasrecommended.
TheantibodywascharacterizedbyimmunohistochemistryandWesternblot.Westernblotshowedasinglebandofapproximately45kDusingadilutionof1/100.Thewesterninformationisincludedasspecificityinformation.Preincubationoftheantibodywithanexcessofthesyntheticpeptideblockedstaining.ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernanalysis,insituhybridizationandreceptorautorADIography.
BlastPdatabasesequencehomologysearchesconfirmedthatthissequenceisuniquetorat,mouseandhuman5-HT1Areceptors.
PhotoDescription:IHCimageofneuronsstainingforthe5-HT1Areceptorintheratcortex(above)and5HT1Areceptorstainingofastrocytesintherathippocampus(below).Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:500for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:Liquid
AvailABIlity: InStock
ReactsWith:GuineaPig,Human,Monkey,Mouse,Rat
AlternateNames: 5-hydroxytryptaminereceptor1A;ADRB2RL1;ADRBRL1;RAT5HT1A,5-hydroxytryptamine(SEROtonin)receptor1A,Gprotein-coupled,anti-5-HT1A
RRID:AB_572210
Immunogen:Rat5-HT1Areceptor(294-312)
GeneSymbol/ID,Accession#: Htr1a,24473
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