Product Name | Beclin 2 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Mouse Beclin 2 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide from the C-terminal of Mouse Beclin 2 (aa. 410-421) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects ~48 kDa. |
Cite This Product | StressMarq Biosciences Cat# SPC-604, RRID: AB_2704781 |
Certificate of Analysis | A 1:1000 dilution of SPC-604 was sufficient for detection of Beclin 2 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Alternative Names | Becn2 Antibody, Beclin-2 Antibody, PFM 2 Antibody, PR domain zinc finger protein 5 Antibody, PRDM 5 Antibody, PRDM5 protein Antibody, PR domain-containing protein 5 Antibody, PRDM5 Antibody, PFM2 Antibody, PR domain containing 5 Antibody, PRDM5_HUMAN Antibody, PR domain containing protein 5 Antibody, BCS2 Antibody |
Research Areas | Cancer, Apoptosis, Autophagy, Cancer Metabolism, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Tumor suppressors |
Cellular Localization | Cytoplasm |
Accession Number | NP_001277621.1 |
Gene ID | 226720 |
Swiss Prot | P0DM65 |
Scientific Background | Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1). |
References | 1. He C., et al. (2013) Cell. 154(5): 1085-1099. |
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Western blot analysis of Mouse Brain cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-604) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
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Properties:
Streptavidin Datasheet
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