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StressMarq/Anti-PDK1 Antibody (pSer241)/SPC-1050D-FITC/100-µl

Overview:

Product Name PDK1 Antibody (pSer241)
Description

Rabbit Anti-Human PDK1 (pSer241) Polyclonal

Species Reactivity Human
Applications WB, AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1 (AA238-244)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects ~63kDa.
Cite This Product StressMarq Biosciences Cat# SPC-1050, RRID: AB_2709299
Certificate of Analysis A 1:250 dilution of SPC-1050 was sufficient for detection of PDK1 (pSer241) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names PDK1 Antibody, PDPK1 Antibody, PDPK1_HUMAN Antibody, PDPK2 Antibody, PkB kinase Antibody, PkB kinase like gene 1 Antibody, PkB like 1 Antibody, PRO0461 Antibody, Protein kinase Antibody3 phosphoinositide dependent protein kinase 1 Antibody, 3-phosphoinositide-dependent protein kinase 1 Antibody, hPDK 1 Antibody, hPDK1 Antibody, MGC20087 Antibody, MGC35290 Antibody, OTTHUMP00000159109 Antibody, OTTHUMP00000159110 Antibody, OTTHUMP00000174525 Antibody
Cellular Localization Cytoplasm, Cell Junction, Cell membrane, Focal Adhesion, Nucleus
Accession Number NP_002601
Gene ID 5170
Swiss Prot O15530

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  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

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