产品资料

StressMarq/Anti-MEK7 Antibody/SPC-1175D-A633/100-µl

Overview:

Product Name MEK7 Antibody

Description

Rabbit Anti-Human MEK7 Polyclonal

Species Reactivity Human, Mouse

Applications WB, AM

Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen Synthetic peptide of human MEK7 (AA370-384)

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Peptide Affinity Purified
Clonality	Polyclonal
Specificity	Detects 47.485 kDa.
Cite This Product	StressMarq Biosciences Cat# SPC-1175, RRID: AB_2711531
Certificate of Analysis	A 1:250 dilution of SPC-1175 was sufficient for detection of MEK7 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	c-Jun N-terminal kinase kinase 2 antibody, Dual specificity mitogen activated protein kinase kinase 7 antibody, JNK activating kinase 2 antibody, JNK kinase 2 antibody, JNKK 2 antibody, Jnkk2 antibody, MAP kinase kinase 7 antibody, MAP2K7 antibody, MAPK/ERK kinase 7 antibody, MAPKK 7 antibody, MEK 7 antibody, Mitogen Activated Protein Kinase kinase 7 antibody, MP2K7_HUMAN antibody, PRKMK 7 antibody, SAPK kinase 4 antibody, SAPKK-4 antibody, SAPKK4 antibody, Sek 2 antibody, Sek-2 antibody, SKK4 antibody, stress-activated protein kinase kinase 4 antibody
Cellular Localization	Cytoplasm, Nucleus
Accession Number	NP_660186
Gene ID	5609
Swiss Prot	O14733
Scientific Background	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

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ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm