产品资料

StressMarq/Anti-PDGFR alpha Antibody (pTyr768)/SPC-1049D-P594/100-µl

Overview:

ProductName PDGFRalphaAntibody(pTyr768)

Description

RabbitAnti-HumanPDGFRalpha(pTyr768)Polyclonal

SpeciesReactivity Human

Applications WB,AM

AntibodyDilution WB(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen Aphospho-specificpeptidecorrespondingtoresiduessurroundingTyr768ofhumanPDGFRAlpha(AA765-771)

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBuffer	PBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	PeptideAffinityPurified
Clonality	Polyclonal
Specificity	Detects122.67kDa.
CiteThisProduct	StressMarqBiosciencesCat#SPC-1049,RRID:AB_2709281
CertificateofAnalysis	A1:250dilutionofSPC-1049wassufficientfordetectionofPDGFRalpha(pTyr768)in10µgof293TcelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames	PDGFalphachainAntibody,PDGFReceptoralphaAntibody,PDGF-R-alphaAntibody,PDGFR2Antibody,PDGFRalphaAntibody,PDGFR-alphaAntibody,PDGFR2Antibody,PDGFRAAntibody,PDGFR-AAntibody,PDGFRA/BCRfusionAntibody,PGFRA_HUMANAntibody,Plateletderivedgrowthfactorreceptor2Antibody,PlateletderivedgrowthfactorreceptoralphaAntibody,PlateletderivedgrowthfactorreceptoralphapolypeptideAntibody,PlateletderivedgrowthfactorreceptorAntibody,Alpha-typeplatelet-derivedgrowthfactorreceptorAntibody,CD140antigen-likefamilymemberAAntibody,CD140aAntibody,CD140aantigenAntibody,MGC74795Antibody,RearrangedinhypereosinophiliaplateletderivedgrowthfactorreceptoralphafusionproteinAntibody,RHEPDGFRAAntibody
CellularLocalization	Cellmembrane,Nucleus
AccessionNumber	NP_006197
GeneID	5156
SwissProt	P16234
ScientificBackground	PDGFRAisareceptor-tyrosinekinasethatisactivateduponbindingplatelet-derivedgrowthfactor(PDGF).LigandbindingcausesdimerizationofthereceptortoactivatesignallingcascadestoactivateeffectorsubstratessuchasPIK3R1,PLCG1,andPTPN11.Effectorsubstrateswillcausereleaseofcalciumfromendoplasmicreticulum,byPLCG1,andactivationofAKT1,byPIK3R1.ThisultimatelyleadstogrowthandproliferationincellsbytheMAPKandSTATtranscriptionfactoractivation.PDGFRAisnegativelyregulatedbyproteinphosphatasestocauseinactivationandinternalization.DuetoPDGFRA"sroleincellulargrowthitsdysfunctionmayleadtumourigenesis.AhighnumberofgastrointestinalstromaltumourshavebeenimplicatedinPDGFRAdysfunctionandconstitutiveactivation.Typicallymis-senseordeletionmutationswerefoundtocauseconstitutivelyactivatedPDGFRA.FurThermore,afusionproteinofPDGFRAwithBCRcanarisefromatranslocationeventwiththePDGFRAgenelocus.Thisfusionproteinhasbeenimplicatedinthedevelopmentofchronicmyeloidleukemias.

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PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm