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TheUCHT1antibodyisspecificforhumanCD3e,alsoknownasCD3epsilon,a20kDasubunitoftheTcellreceptorcomplex,alongwithCD3gammaandCD3delta.TheseintegralmembraneproteinchainsassemblewithadditionalchainsoftheTcellreceptor(TCR),aswellasCD3zetachain,toformtheTcellreceptor–CD3complex.Togetherwithco-receptorsCD4orCD8,thecomplexservestorecognizeantigensboundtoMHCmoleculesonantigen-presentingcells.TheseinteractionspromoteTcellreceptorsignaling(Tcellactivation),inducingcellproliferation,differentiation,productionofcytokinesoractivation-inducedcelldeath.CD3isdifferentiallyexpressedduringthymocyte-to-TcelldevelopmentandonallmatureTcells.
TheUCHT1antibodyisawidelyusedphenotypicmarkerforhumanTcells.Inaddition,binding/cross-linkingofUCHT1antibodytoCD3ecaninducecellactivation(useformatsuitableforfunctionalassays).ArecentpublicationofthecrystalstructureofaCD3e-antibodycomplexprovidesinsightastotheactionofcommonlyusedagonistantibodies,aswellasspecificepitope-bindingdataforthehumanCD3antibodiesUCHT1andOKT3(Fernandes,R.A.etal.2012.J.Biol.Chem.287:13324-13335).
UCHT1antibodyreactswithbothsurface-expressedandintracellularCD3eprotein,incontrasttoanalternativehumanCD3clone,Hit3a,whichwillstainonlytheextracellular(membrane-expressed)CD3eprotein.Also,theUCHT1antibodyisreportedtobecross-reactivewithchimpanzeeandhasbeenusedforphenotypicanalysisofexpressionbyflowcytometry;howevertheantibodyisreportedtobeunsuitableforinductionofTcellactivationinthisspecies(Bibollet-Rucheetal.2009.J.Virol.82:10271-10278).
| Name | APCAnti-HumanCD3(UCHT1) |
|---|---|
| Cat.No. | 20-0038 |
| AlternativeNames | Leu-4,T3 |
| GeneID | 915 |
| Clone | UCHT1 |
| Isotype | MouseIgG1,kappa |
| Reactivity | Human |
| CrossReactivity | Chimpanzee |
| Format | APC |
| Applications | FlowCytometry |
| Citations* | HarrisSJ,ParryRV,FosterJG,BluntMD,WangA,Marelli-BergF,WestwickJ,andWardSG.Apr.2011.J.Immunol.186:4936-4945.(invitroactivation) BeriouG,BradshawEM,LozanoE,CostantinoCM,HastingsWD,OrbanT,ElyamanW,KhourySJ,KuchrooVK,Baecher-AllanC,andHaflerDA.2010.J.Immunol.185:46-54.(invitroactivation) SotoPC,SteinLL,Hurtado-ZiolaN,HedrickSM,andVarkiA.2010.J.Immunol.184:4185-4195.(Flowcytometry–Chimpanzee) EdelbauerM,DattaD,VosIHC,BasuA,StackMP,ReindersMEJ,ShoM,CalzadillaK,GanzeP,andBriscoeDM.2010.Blood.116:1980-1989.(Immunohistochemistry–acetonefixed,frozensections;Immunofluoresencemicroscopy) VargheseJCandKaneKP.2008.J.Immunol.181:6002-6009.(invitroactivation) MackCL,TuckerRM,SokolRJ,DarrerFM,KotzinBL,WhitingtonPFandMillerSD.2004.Pediatr.Res.56(1):79-87.(Immunohistochemistry–frozentissue) SakkasLI,ScanzelloC,JohansonN,BurkholderJ,MitraA,SalgameP,KatsetosCD,andPlatsoucasCD.1998.Clin.Diagn.Lab.Immun.5:430.(Immunohistochemistry–acetonefixed,frozensections) SalmeronA,Sanchez-MadridF,UrsaMA,FresnoM,andAlarconB.1991.J.Immunol.147:3047-3052.(Immunoprecipitation) VanDongenJJ,KrissansenGW,Wolvers-TetteroIL,Comans-BitterWM,AdriaansenHJ,HooijkaasH,vanWeringER,andTerhorstC.1988.Blood.71:603-612.(WesternBlot) |